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Journal / Immunology and Infection / Proliferasyon ınsan antijeni spesifik CD4+ T…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Proliferasyon ınsan antijeni spesifik CD4+ T hücrelerinin Karboksflorcein Succinimidyl Ester kullanarak ölçülmesini
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Proliferasyon ınsan antijeni spesifik CD4+ T hücrelerinin Karboksflorcein Succinimidyl Ester kullanarak ölçülmesini

Article DOI: 10.3791/59545-v • 07:00 min • June 4th, 2019
June 4th, 2019 •
1, 1, 1, 1,2
1Immunology and Diabetes Unit, St. Vincent's Institute of Medical Research, 2Department of Medicine, University of Melbourne, St. Vincent's Hospital

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Burada sunulan bir protokol Proliferasyona CD4+ T hücreleri antijenik proteinler veya peptidler boya seyreltme kullanarak yanıt olarak. Bu tahlil nadir antijen özgü T hücrelere özellikle duyarlıdır ve antijen özgü hücrelerin klonlama kolaylaştırmak için değiştirilebilir.

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İmmünoloji ve enfeksiyon Sayı 148 insan CD4 proliferasyon CFSE hassas otoantijen klonlama PBMC
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