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Journal / Genetics / 从小鼠横纹肌瘤分离的原发性肿瘤细胞的肿瘤球衍生和治疗…
JoVE Journal
Genetics

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从小鼠横纹肌瘤分离的原发性肿瘤细胞的肿瘤球衍生和治疗
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从小鼠横纹肌瘤分离的原发性肿瘤细胞的肿瘤球衍生和治疗

Article DOI: 10.3791/59897-v • 09:21 min • September 13th, 2019
September 13th, 2019 •
1,2,3, 2
1Graduate School of Biomedical Sciences, Sanford Burnham Prebys Medical Discovery Institute, 2Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, 3Department of Obstetrics, Gynecology, and Reproductive Sciences, Division of Female Pelvic Medicine and Reconstructive Surgery, University California San Diego

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Summary
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该协议描述了一种从肿瘤层培养开始分离小鼠横纹肌瘤原发细胞、肿瘤球的形成和治疗以及异体移植的可重复方法。

Tags

遗传学， 问题 151， 肿瘤球， 横纹肌瘤， 骨骼肌， 原发细胞， 重组蛋白治疗， 质粒转染
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