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Immunology and Infection

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JoVE Journal Immunology and Infection
Trasfezione altamente efficiente dei macrofagi primari con mRNA trascritto in vitro
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Trasfezione altamente efficiente dei macrofagi primari con mRNA trascritto in vitro

Article DOI: 10.3791/60143-v • 06:46 min • November 9th, 2019
November 9th, 2019 •
1, 1, 1, 1,2,3,4, 1
1Institute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne, University of Cologne, 2Center for Molecular Medicine Cologne, 3Cologne Cluster of Excellence on Cellular Stress Responses in Aging-associated Diseases (CECAD), 4German Center for Infection Research (DZIF)

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I macrofagi, in particolare i macrofagi primari, sono difficili da trasfecare in quanto sono specializzati nel rilevamento di molecole di origine non auto. Descriviamo un protocollo che consente una trasfezione altamente efficiente dei macrofagi primari con mRNA generati da modelli di DNA come i plasmidi.

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Immunologia e infezione numero 153 macrofagi primari macrofagi peritoneali macrofagi derivati dal midollo osseo cellule immunitarie trascrizione in vitro mRNA trasfezione
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