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Journal / Immunology and Infection / In Vitro Transcribed mRNA ile Primer…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
In Vitro Transcribed mRNA ile Primer Makrofajların Yüksek Verimli Transföytüsü
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In Vitro Transcribed mRNA ile Primer Makrofajların Yüksek Verimli Transföytüsü

Article DOI: 10.3791/60143-v • 06:46 min • November 9th, 2019
November 9th, 2019 •
1, 1, 1, 1,2,3,4, 1
1Institute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne, University of Cologne, 2Center for Molecular Medicine Cologne, 3Cologne Cluster of Excellence on Cellular Stress Responses in Aging-associated Diseases (CECAD), 4German Center for Infection Research (DZIF)

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Makrofajlar, özellikle birincil makrofajlar, kendi kendine kökenli olmayan molekülleri tespit etmede uzmanlaşdıkları için transfect yapmak zordur. Plazmidler gibi DNA şablonlarından oluşturulan mRNA ile birincil makrofajların yüksek verimli transfeksiyonuna olanak tanıyan bir protokolü tanımlıyoruz.

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İmmünoloji ve Enfeksiyon Sayı 153 Primer makrofajlar periton makrofajları kemik iliği kaynaklı makrofajlar bağışıklık hücreleri in vitro transkripsiyon mRNA transfeksiyon
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