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Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles
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Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles

Article DOI: 10.3791/60596-v • 09:30 min • March 31st, 2020
March 31st, 2020 •
1, 1, 1, 1, 1
1Department of Pathology, University of Washington

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Summary
Automatic Translation

This article presents methods for generating, purifying, and quantifying Caenorhabditis elegans extracellular vesicles.

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Caenorhabditis Elegans Extracellular Vesicles Purification Analysis Genetically Tractable Invertebrate Model Protein Signals RNA Signals Protocol RNA-SEQ Proteomic Analysis Synchronized Culture Food Exhaustion Rubber Bands Nylon Mesh Filter Cap Vacuum L1 Worms Medium Volume Worm Aggregates Vortexing Worm Cultivation Plate Lid Animal Count Fresh Medium Mean And Standard Error Of The Mean Worm Population Percentage Total Number Of Animals Secretome Generation Washes
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