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Journal / Biochemistry / Induktion af Eryptose i røde blodlegemer ved…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Induktion af Eryptose i røde blodlegemer ved hjælp af en calcium Ionophore
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Induktion af Eryptose i røde blodlegemer ved hjælp af en calcium Ionophore

Article DOI: 10.3791/60659-v • 09:15 min • January 21st, 2020
January 21st, 2020 •
1, 1,2
1Biomedical Engineering Program, Ohio University, 2Department of Chemical and Biomolecular Engineering, Ohio University

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En protokol til induktion af eryptose, programmeret celledød i erythrocytter, ved hjælp af calcium ionophore, ionomycin, er tilvejebragt. Vellykket eryptose evalueres ved at monitorere lokaliseringen phosphatidylserin i membranen ydre folder. Faktorer, der påvirker protokollens succes, er blevet undersøgt, og de optimale betingelser er opfyldt.

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Biokemi erythrocyt eryptose phosphatidylserin bilagin V ionomycin celle membran
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