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Journal / Biology / Ultrayapısal Olarak Korunmuş Hücrelerde Membran…
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Biology

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JoVE Journal Biology
Ultrayapısal Olarak Korunmuş Hücrelerde Membran Protein Lokalizasyonunun Elektron Mikroskobu Analizi için CryoAPEX Yöntemi
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Ultrayapısal Olarak Korunmuş Hücrelerde Membran Protein Lokalizasyonunun Elektron Mikroskobu Analizi için CryoAPEX Yöntemi

Article DOI: 10.3791/60677-v • 11:45 min • February 27th, 2020
February 27th, 2020 •
1, 2, 2,3,4, 1,4,5,6
1Department of Biological Sciences, Purdue University, 2Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, 3Purdue Institute of Inflammation, Immunology and Infectious Disease, Purdue University, 4Bindley Biosciences Center, Purdue University, 5Purdue Institute of Integrative Neuroscience, Purdue University, 6Purdue Center for Cancer Research, Purdue University

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Bu protokol, apex2 etiketli membran proteininin en iyi korunmuş hücre ultrayapısı içinde iletim elektron mikroskobu ile lokalize edilebildiği kriyoAPEX yöntemini açıklar.

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Biyoloji Sayı 156 CryoAPEX membran protein APEX2 iletim elektron mikroskobu cryofixation yüksek basınç dondurma dondurma ikamesi
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