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Journal / Immunology and Infection / Reverse Transcription Loop-Bemiddelde Isothermale …
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Reverse Transcription Loop-Bemiddelde Isothermale Versterking (RT-LAMP) Assay voor de specifieke en snelle detectie van Tilapia Lake Virus
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Reverse Transcription Loop-Bemiddelde Isothermale Versterking (RT-LAMP) Assay voor de specifieke en snelle detectie van Tilapia Lake Virus

Article DOI: 10.3791/61025-v • 07:47 min • May 18th, 2020
May 18th, 2020 •
1, 2,3, 2, 4, 2,3
1Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok (KMUTNB), 2Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, 3Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, 4Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS), King Mongkut's University of Technology North Bangkok (KMUTNB)

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We presenteren een RT-LAMP test voor de detectie van TiLV in tilapia vissen met behulp van eenvoudige instrumenten over een relatief korte periode van tijd in vergelijking met conventionele RT-PCR technieken. Dit protocol kan helpen bij de bestrijding van de epidemische verspreiding van TiLVD, vooral in ontwikkelingslanden.

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Immunologie en infectie nummer 159 tilapia lake virus diagnose tilapia RT-LAMP RT-qPCR screening tool
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