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Journal / Biology / 포토컨버터블 덴드라2를 사용하여 노화 C. 엘레간스의 단백질 회전율의 생체 내…
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Biology

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JoVE Journal Biology
포토컨버터블 덴드라2를 사용하여 노화 C. 엘레간스의 단백질 회전율의 생체 내 정량화
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포토컨버터블 덴드라2를 사용하여 노화 C. 엘레간스의 단백질 회전율의 생체 내 정량화

Article DOI: 10.3791/61196-v • 09:45 min • June 13th, 2020
June 13th, 2020 •
1,2, 1,3
1Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund, 2NeuroCure Cluster of Excellence, Charité - Universitätsmedizin Berlin, 3Department of Biology and Chemistry, University of Bremen

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여기에 제시된 단백질 헌팅틴의 분해를 광변환형 형광소 덴드라2에 융합한 분해를 모니터링하는 프로토콜이 있다.

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생물학 문제 160 C. elegans,헌팅틴 프로테오스타증 네트워크 저하 Dendra2 광변환 공초점 현미경 검사 피지/이미지J
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