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Journal / Cancer Research / In vivo Triagem de imunogenicidade de vesículas…
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Cancer Research

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In vivo Triagem de imunogenicidade de vesículas extracelulares derivadas do tumor por citometria de fluxo de células T esplênicas
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In vivo Triagem de imunogenicidade de vesículas extracelulares derivadas do tumor por citometria de fluxo de células T esplênicas

Article DOI: 10.3791/62811-v • 08:02 min • September 23rd, 2021
September 23rd, 2021 •
1,2, 1,2,3,4, 1,2
1Department of Medicine III, School of Medicine, Technical University of Munich, 2Center for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of Munich, 3Department of Internal Medicine III, University Hospital Regensburg, 4National Centre for Tumor Diseases WERA

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Este manuscrito descreve como avaliar a imunogenicidade in vivo de vesículas extracelulares derivadas de células tumorais (EVs) usando citometria de fluxo. Os EVs derivados de tumores submetidos à morte de células imunogênicas induzidas pelo tratamento parecem particularmente relevantes na imunossuperveiência tumoral. Este protocolo exemplifica a avaliação dos EVs de tumor imunoestimulatório induzidos por oxaliplatina, mas pode ser adaptado a várias configurações.

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Pesquisa sobre câncer edição 175
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