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Biology
Biology
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从成年小鼠肾脏中分离细胞核以进行单核RNA测序
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Article
DOI:
10.3791/62901-v
•
06:00 min
•
September 20th, 2021
从成年小鼠肾脏中分离细胞核以进行单核RNA测序
September 20th, 2021
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Janna Leiz*1,2, Christian Hinze*1,2, Anastasiya Boltengagen3, Caroline Braeuning4, Christine Kocks3, Nikolaus Rajewsky3, Kai M. Schmidt-Ott1,2
1Department of Nephrology and Medical Intensive Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 2Molecular and Translational Kidney Research, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 3Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 4Berlin Institute of Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)
* These authors contributed equally
Kapitel
Summary
Please note that all translations are automatically generated.
Click here for the English version.
在这里,我们提出了一种从冷冻小鼠肾脏中分离高质量细胞核的方案,该方案改善了髓质肾细胞类型的表征,并避免了酶组织解离引起的基因表达伪像。