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Journal / Biochemistry / 肝糖原分子的提取用于糖原结构测定
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
肝糖原分子的提取用于糖原结构测定
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
Journal (Biochemistry)
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
Journal (Biochemistry)
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
 
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肝糖原分子的提取用于糖原结构测定

Article DOI: 10.3791/63088-v • 04:50 min • February 8th, 2022
February 8th, 2022 •
1,2, 3,4, 3,5, 1,2,6, 7
1School of Chemistry and Molecular Biosciences, The University of Queensland, 2Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, 3Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, 4Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical University, 5Department of Bioinformatics, School of Medical Informatics and Engineering, Xuzhou Medical University, 6Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture, Yangzhou University, 7Glycation and Diabetes Group, Mater Research Institute-The University of Queensland, Translational Research Institute

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使用蔗糖密度梯度离心法确定提取肝糖原的最佳蔗糖浓度。加入 10 分钟的煮沸步骤以抑制糖原降解酶被证明是有益的。

Tags

本月在JoVE第180期，糖原结构测定，葡萄糖聚合物，血糖水平，糖原的性质，提取方法，蔗糖密度梯度离心，分子损伤，蔗糖溶液密度，糖原颗粒大小，煮沸步骤，变性糖原降解酶
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