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Journal / Neuroscience / Registrering av kalsiumtransienter i mus…
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Neuroscience

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JoVE Journal Neuroscience
Registrering av kalsiumtransienter i mus Neuromuscular Junction med høy temporal oppløsning ved bruk av konfokal mikroskopi
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Registrering av kalsiumtransienter i mus Neuromuscular Junction med høy temporal oppløsning ved bruk av konfokal mikroskopi

Article DOI: 10.3791/63308-v • 11:12 min • December 1st, 2021
December 1st, 2021 •
1, 1, 1,2, 3, 1,2
1Laboratory of Biophysics of Synaptic Processes, Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, 2Department of Radiophotonics and Microwave Technologies, Kazan National Research Technical University named after A.N. Tupolev, 3Department of Normal Physiology, Kazan State Medical University

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Protokollen beskriver metoden for å laste et fluorescerende kalsiumfargestoff gjennom kuttnerven i musemotoriske nerveterminaler. I tillegg presenteres en unik metode for registrering av raske kalsiumtransienter i perifere nerveender ved hjelp av konfokalmikroskopi.

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