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Journal / Biochemistry / Dekryptere molekylær mekanisme for…
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Biochemistry

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Dekryptere molekylær mekanisme for histonmontering ved DNA-gardinteknikk
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Dekryptere molekylær mekanisme for histonmontering ved DNA-gardinteknikk

Article DOI: 10.3791/63501-v • 06:32 min • March 9th, 2022
March 9th, 2022 •
1, 1, 1, 1,2
1Department of Biological Sciences, Ulsan National Institute of Science and Technology, 2Center for Genomic Integrity, Institute for Basic Science (IBS)

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DNA-gardin, en high-throughput enkeltmolekylær bildebehandlingsteknikk, gir en plattform for sanntidsvisualisering av ulike protein-DNA-interaksjoner. Den nåværende protokollen benytter DNA-gardinteknikken for å undersøke den biologiske rollen og molekylære mekanismen til Abo1, en Schizosaccharomyces pombe bromdomene som inneholder AAA + ATPase.

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Molekylær mekanisme histonmontering DNA-gardinteknikk kromatin eukaryot DNA dynamiske endringer cellesyklusfase miljøstimuli genomisk integritet epigenetisk regulering DNA-metabolske reaksjoner replikasjon transkripsjon reparasjon histonchaperoner nukleosomer enkeltmolekylavbildningsteknikk DNA-gardin lipidfluiditet mikrofluidikk total intern refleksjonsfluorescensmikroskopi (TIRFM) protein-DNA-interaksjoner Abo1 Schizosaccharomyces pombe Bromdomene-inneholdende AAA+ ATPase
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