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Eksperimentell melanom immunterapimodell ved bruk av tumorvaksinasjon med et hematopoietisk cytokin
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Eksperimentell melanom immunterapimodell ved bruk av tumorvaksinasjon med et hematopoietisk cytokin

Article DOI: 10.3791/64082-v • 09:15 min • February 24th, 2023
February 24th, 2023 •
1, 1, 1, 1
1La Jolla Institute for Immunology

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Protokollen presenterer en kreftimmunterapimodell ved bruk av cellebasert tumorvaksinasjon med Flt3L-uttrykkende B16-F10 melanom. Denne protokollen demonstrerer prosedyrene, inkludert fremstilling av dyrkede tumorceller, tumorimplantasjon, cellebestråling, måling av tumorvekst, isolering av intratumorale immunceller og flowcytometrianalyse.

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Kreftforskning utgave 192 Flt3L DC B16-F10 melanom tumorvaksine medfødt immunitet intratumorale immunceller
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