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Journal / Neuroscience / 使用MRI，组织清除和光片显微镜对完整的新生小鼠大脑进行全脑单细胞成像和分析…
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Neuroscience

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JoVE Journal Neuroscience
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使用MRI，组织清除和光片显微镜对完整的新生小鼠大脑进行全脑单细胞成像和分析

Article DOI: 10.3791/64096-v • 08:49 min • August 1st, 2022
August 1st, 2022 •
*1,2, *1,2, 1,2, 1,2, 3, 3,7, 7, 4,5,6, 4,5,6, 3, 4,5,6, 1,2
1UNC Neuroscience Center, University of North Carolina, Chapel Hill, 2Department of Genetics, University of North Carolina, Chapel Hill, 3Department of Psychiatry, University of North Carolina, Chapel Hill, 4Center for Animal Magnetic Resonance Imaging, The University of North Carolina at Chapel Hill, 5Biomedical Research Imaging Center, The University of North Carolina at Chapel Hill, 6Department of Neurology, The University of North Carolina at Chapel Hill, 7Department of Computer Science, The University of North Carolina at Greensboro
* These authors contributed equally

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Summary
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该协议描述了使用iDISCO +对完整小鼠大脑进行磁共振成像，清除和免疫标记的方法，然后详细说明了使用光片显微镜成像和使用NuMorph的下游分析。

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神经科学，第186期，
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