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JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

MRI, 조직 투명화 및 라이트 시트 현미경을 사용한 온전한 신생아 마우스 뇌의 전뇌 단일 세포 이미징 및 분석

Using Zebrafish Larvae to Study the Pathological Consequences of Hemorrhagic Stroke

Journal (Neuroscience)

Using Zebrafish Larvae to Study the Pathological Consequences of Hemorrhagic Stroke

Quantitative Analysis of Climbing Defects in a Drosophila Model of Neurodegenerative Disorders

Journal (Neuroscience)

Quantitative Analysis of Climbing Defects in a Drosophila Model of Neurodegenerative Disorders

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MRI, 조직 투명화 및 라이트 시트 현미경을 사용한 온전한 신생아 마우스 뇌의 전뇌 단일 세포 이미징 및 분석

Article DOI: 10.3791/64096-v • 08:49 min • August 1st, 2022

August 1st, 2022 •

Felix A. Kyere*^1,2, Ian Curtin*^1,2, Oleh Krupa^1,2, Carolyn M. McCormick^1,2, Mustafa Dere³, Sarah Khan^3,7, Minjeong Kim⁷, Tzu-Wen Winnie Wang^4,5,6, Qiuhong He^4,5,6, Guorong Wu³, Yen-Yu Ian Shih^4,5,6, Jason L. Stein^1,2

¹UNC Neuroscience Center, University of North Carolina, Chapel Hill, ²Department of Genetics, University of North Carolina, Chapel Hill, ³Department of Psychiatry, University of North Carolina, Chapel Hill, ⁴Center for Animal Magnetic Resonance Imaging, The University of North Carolina at Chapel Hill, ⁵Biomedical Research Imaging Center, The University of North Carolina at Chapel Hill, ⁶Department of Neurology, The University of North Carolina at Chapel Hill, ⁷Department of Computer Science, The University of North Carolina at Greensboro

* These authors contributed equally

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이 프로토콜은 iDISCO+를 사용하여 온전한 마우스 뇌의 자기 공명 영상, 제거 및 면역 표지를 수행하는 방법을 설명한 다음, 라이트 시트 현미경을 사용한 이미징 및 NuMorph를 사용한 다운스트림 분석에 대한 자세한 설명을 설명합니다.

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