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Journal / Biochemistry / Studio delle funzioni e delle attività del…
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Biochemistry

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Studio delle funzioni e delle attività del co-trasportatore neuronale K-Cl KCC2 mediante Western Blotting
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Studio delle funzioni e delle attività del co-trasportatore neuronale K-Cl KCC2 mediante Western Blotting

Article DOI: 10.3791/64179-v • 10:08 min • December 9th, 2022
December 9th, 2022 •
1, 1, 1, 1
1Institute of Biomedical and Clinical Sciences, Medical School, Faculty of Health and Life Sciences, University of Exeter, Hatherly Laboratories

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Il presente protocollo evidenzia l'applicazione della tecnica western blotting per studiare le funzioni e le attività del co-trasportatore neuronale K-Cl KCC2. Il protocollo descrive lo studio della fosforilazione di KCC2 nei siti regolatori delle chinasi Thr906/1007 tramite western blotting. Inoltre, ulteriori metodi per confermare l'attività di KCC2 sono brevemente evidenziati in questo testo.

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Biochimica Numero 190
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