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Immunology and Infection

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Isolamento Fecal Murino e Transplante de Microbiota

Article DOI: 10.3791/64310-v • 07:32 min • May 26th, 2023
May 26th, 2023 •
1, 2,3, 2, 1
1Division of Clinical Pharmacology, Department of Medicine Vanderbilt University Medical Center and Department of Molecular Physiology and Biophysics, Vanderbilt University, 2Division of Pediatric Nephrology Department of Pediatrics, Vanderbilt University Medical Center, 3Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center

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O objetivo aqui é delinear um protocolo para investigar os mecanismos de disbiose na doença cardiovascular. Este artigo discute como coletar e transplantar amostras fecais murinas assepticamente, isolar intestinos e usar o método "Swiss-roll", seguido por técnicas de imunomarcação para interrogar alterações no trato gastrointestinal.

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Imunologia e Infecção Edição 195 transplante de microbiota fecal Swiss-roll cardiovascular
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