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A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

DOI:
10.3791/64582-v

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08:19 min

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December 16, 2022

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, , , , , , , ,
1Department of Developmental Biology,University of Pittsburgh, 2Department of Biological Sciences,University of Pittsburgh, 3Département de Biologie,École Normale Supérieure de Lyon, 4Department of Critical Care Medicine,University of Pittsburgh

Kapitel

  • 00:05Introduction
  • 01:05Fetal Echocardiogram
  • 01:46Necropsy
  • 02:41Episcopic Confocal Microscopy (ECM)
  • 04:01Three-Dimensional (3D) Reconstruction
  • 06:14Results: Analyzing Cardiac Anomalies Using Echo and ECM
  • 07:19Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This article details murine congenital heart disease (CHD) diagnostic methods using fetal echocardiography, necropsy, and Episcopic fluorescence image capture (EFIC) using Episcopic confocal microscopy (ECM) followed by three-dimensional (3D) reconstruction.

Tags

Fetal MouseCongenital Heart DefectsEchocardiographyNecropsyConfocal MicroscopyAnatomical ChangesStructural Birth DefectsUltrasoundColor DopplerSample PreparationLaser Confocal ImagingImage Acquisition

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