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JoVE Journal Developmental Biology
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A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

Article DOI: 10.3791/64582-v • 08:19 min • December 16th, 2022
December 16th, 2022 •
*1, *2, *1, *3, *1, 2, 2, 1, 1,4
1Department of Developmental Biology, University of Pittsburgh, 2Department of Biological Sciences, University of Pittsburgh, 3Département de Biologie, École Normale Supérieure de Lyon, 4Department of Critical Care Medicine, University of Pittsburgh
* These authors contributed equally

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This article details murine congenital heart disease (CHD) diagnostic methods using fetal echocardiography, necropsy, and Episcopic fluorescence image capture (EFIC) using Episcopic confocal microscopy (ECM) followed by three-dimensional (3D) reconstruction.

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Structural Heart Defects Fetal Mouse High Resolution Imaging Congenital Heart Defects Fetal Echocardiography Necropsy Confocal Microscopy Histopathology 2D Image Stacks 3D Reconstructions Anatomical Changes Cranial Facial Defects Limb And Skeletal Anomalies Brain Defects Visceral Organ Defects Ultrasound Gel Transducer Bladder Identification Gestational Age Measurement Color Doppler Analysis Fixative Penetration
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