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JoVE Journal Neuroscience
Anrikning av voksne mus Dorsal Root Ganglia Neuron Kulturer ved Immunopanning
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Anrikning av voksne mus Dorsal Root Ganglia Neuron Kulturer ved Immunopanning

Article DOI: 10.3791/64603-v • 08:57 min • February 24th, 2023
February 24th, 2023 •
1, 1,2,3, 1,4,5
1Neuroscience and Mental Health Institute, University of Alberta, 2Department of Medicine, Division of Neurology, University of Alberta, 3Department of Medical Microbiology and Immunology, University of Alberta, 4Department of Pharmacology, University of Alberta, 5Department of Anesthesiology and Pain Medicine, University of Alberta

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Denne artikkelen beskriver en immunpaneringsprotokoll for voksne dorsale rotganglier hos mus. Ved å feste antistoffer mot kulturplater, kan vi negativt velge og fjerne ikke-nevronale celler. Vi viser at kulturene er beriket for nevroner ved hjelp av denne protokollen, noe som muliggjør en grundig studie av nevronresponser på manipulasjon.

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