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Arricchimento di colture di neuroni dei gangli dorsali di topo adulto mediante immunopanning
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Arricchimento di colture di neuroni dei gangli dorsali di topo adulto mediante immunopanning

Article DOI: 10.3791/64603-v • 08:57 min • February 24th, 2023
February 24th, 2023 •
1, 1,2,3, 1,4,5
1Neuroscience and Mental Health Institute, University of Alberta, 2Department of Medicine, Division of Neurology, University of Alberta, 3Department of Medical Microbiology and Immunology, University of Alberta, 4Department of Pharmacology, University of Alberta, 5Department of Anesthesiology and Pain Medicine, University of Alberta

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Questo articolo descrive un protocollo di immunopanning per i gangli della radice dorsale del topo adulto. Aderendo agli anticorpi alle piastre di coltura, possiamo selezionare e rimuovere negativamente le cellule non neuronali. Mostriamo che le colture sono arricchite per i neuroni utilizzando questo protocollo, consentendo uno studio approfondito delle risposte neuronali alla manipolazione.

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Ritrattazione numero 192
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