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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

フローサイトメトリー法 による 日本脳炎ワクチン接種小児における多機能性T細胞の検出

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs

Journal (Immunology and Infection)

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs

Production of Human CRISPR-Engineered CAR-T Cells

Journal (Immunology and Infection)

Production of Human CRISPR-Engineered CAR-T Cells

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フローサイトメトリー法による日本脳炎ワクチン接種小児における多機能性T細胞の検出

Article DOI: 10.3791/64671-v • 09:37 min • September 23rd, 2022

September 23rd, 2022 •

Linlin Zhang*^1,2, Meng Zhang*^1,2,3, Mengjia Liu^1,2, Junhong Ai^1,2, Jiao Tian^1,2, Huizheng Ge⁴, Ran Wang^1,2, Zhengde Xie^1,2

¹Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, ²Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences, ³Department of Pediatric Rehabilitation, Beijing Boai Hospital, School of Rehabilitation Medicine, Capital Medical University, China Rehabilitation Research Center, ⁴Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd

* These authors contributed equally

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Click here for the English version.

本プロトコルは、 ex vivo 刺激とフローサイトメトリーを組み合わせて、日本脳炎ウイルス(JEV)ワクチン接種小児内の末梢血単核球(PBMC)の多機能T細胞(_TFP)プロファイルを分析します。JEV特異的TPFの検出方法とフローサイトメトリーの配色を試験し、同様の研究の参考資料を提供しました。

Tags

免疫感染症学、第187号、多機能T細胞、日本脳炎、ワクチン接種、フローサイトメトリー

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