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Cancer Research

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JoVE Journal Cancer Research

نمذجة سرطان الخلايا الحرشفية الفموية المريئية في عضويات 3D

Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol

Journal (Cancer Research)

Moving Upwards: A Simple and Flexible In Vitro Three-dimensional Invasion Assay Protocol

Generation of a Liver Orthotopic Human Uveal Melanoma Xenograft Platform in Immunodeficient Mice

Journal (Cancer Research)

Generation of a Liver Orthotopic Human Uveal Melanoma Xenograft Platform in Immunodeficient Mice

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نمذجة سرطان الخلايا الحرشفية الفموية المريئية في عضويات 3D

Article DOI: 10.3791/64676-v • 10:43 min • December 23rd, 2022

December 23rd, 2022 •

Samuel Flashner*¹, Cecilia Martin*^1,2, Norihiro Matsuura¹, Masataka Shimonosono¹, Yasuto Tomita¹, Masaki Morimoto¹, Ogoegbunam Okolo¹, Victoria X. Yu^1,3, Anuraag S. Parikh^1,3, Andres J. P. Klein-Szanto⁴, Kelley Yan^1,2, Joel T. Gabre^1,5, Chao Lu^1,6, Fatemeh Momen-Heravi^1,7, Anil K. Rustgi^1,5, Hiroshi Nakagawa^1,2,5

¹Herbert Irving Comprehensive Cancer Center, Columbia University, ²Organoid and Cell Culture Core, Columbia University Digestive and Liver Diseases Research Center, Columbia University, ³Department of Otolaryngology, Head and Neck Surgery, Columbia University, ⁴Histopathology Facility, Fox Chase Cancer Center, ⁵Division of Digestive and Liver Diseases, Department of Medicine, Columbia University, ⁶Department of Genetics and Development, Columbia University, ⁷Section of Oral, Diagnostic and Rehabilitation Sciences, College of Dental Medicine, Columbia University

* These authors contributed equally

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يصف هذا البروتوكول الخطوات الرئيسية لتوليد وتوصيف عضويات 3D عن طريق الفم والمريء التي تمثل آفات سرطان الخلايا الطبيعية والأورام والحرشفية الناجمة عن التسرطن الكيميائي.

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أبحاث السرطان ، العدد 190 ،

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