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Bioengineering

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Rapid Development of Cell State Identification Circuits with Poly-Transfection

Article DOI: 10.3791/64793-v • 09:21 min • February 24th, 2023
February 24th, 2023 •
1, 1,2, 3, 1,3
1Department of Biological Engineering, Massachusetts Institute of Technology, 2School of Biological Engineering, University of British Columbia, 3Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology

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Summary
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Complex genetic circuits are time-consuming to design, test, and optimize. To facilitate this process, mammalian cells are transfected in a way that allows the testing of multiple stoichiometries of circuit components in a single well. This protocol outlines the steps for experimental planning, transfection, and data analysis.

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Bioengineering Biological Parts Ratio Metric Combinations Circuit Components Synthetic Biology Flow Cytometry DNA Aggregates Reduced Serum Medium Filler Plasmid Color Control Tubes
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