High-Quality Seizure-Like Activity from Acute Brain Slices Using a Complementary Metal-Oxide-Semiconductor High-Density Microelectrode Array System

Melissa L. Blotter; Isaac W. Stubbs; Jacob H. Norby; Maxwell Holmes; Ben Kearsley; Alexis Given; Kutter Hine; Micah R. Shepherd; R. Ryley Parrish

doi:10.3791/67065

Hochwertige anfallsähnliche Aktivität aus akuten Hirnschnitten unter Verwendung eines komplementä...

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Neuroscience

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JoVE Journal Neuroscience

High-Quality Seizure-Like Activity from Acute Brain Slices Using a Complementary Metal-Oxide-Semiconductor High-Density Microelectrode Array System

Hochwertige anfallsähnliche Aktivität aus akuten Hirnschnitten unter Verwendung eines komplementären Metalloxid-Halbleiter-Mikroelektrodenarray-Systems mit hoher Dichte

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06:28 min

September 27, 2024

DOI: 10.3791/67065-v

Melissa L. Blotter*^1,2, Isaac W. Stubbs*^1,2, Jacob H. Norby*^1,2, Maxwell Holmes^1,2, Ben Kearsley³, Alexis Given¹, Kutter Hine^1,4, Micah R. Shepherd⁵, R. Ryley Parrish^1,2

¹Department of Cell Biology and Physiology,Brigham Young University, ²Neuroscience Center,Brigham Young University, ³Department of Statistics,Brigham Young University, ⁴Department of Biology,Brigham Young University, ⁵Department of Physics and Astronomy,Brigham Young University

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Overview

This study outlines a protocol for using complementary metal-oxide-semiconductor high-density microelectrode array systems (CMOS-HD-MEAs) to investigate seizure-like activity from ex vivo brain slices. The research aims to enhance the understanding of seizure initiation, propagation, and termination, with a focus on developing novel therapies for status epilepticus.

Key Study Components

Area of Science

Neuroscience
Electrophysiology
Epileptology

Background

Seizures can have complex initiation and propagation mechanisms.
Status epilepticus is a critical condition that often resists treatment.
High-density microelectrode arrays provide detailed electrophysiological data.
Different paradigms offer insights into the dynamics of seizure-like activity.

Purpose of Study

To establish a reliable protocol for recording seizure activity.
To explore the spatial and temporal patterns of status epilepticus.
To inform the development of more effective treatments for severe seizures.

Methods Used

The main platform used is CMOS-HD-MEAs with ex vivo brain slices.
Brain slices are subjected to various treatment paradigms to investigate seizure-like activity.
Detailed preparation and maintenance steps are provided for optimal recordings.
The protocol includes steps for preparing the microelectrode array and ensuring proper environmental conditions.
Electrophysiological activity is recorded to analyze seizure characteristics.

Main Results

Neocortical regions show significant electrographic seizure-like activity under specific conditions.
Hippocampal regions exhibited variability in seizure-like activity.
Different paradigms revealed distinct power dynamics in seizure frequencies.

Conclusions

This study facilitates a deeper understanding of seizure mechanisms using advanced technology.
The findings may contribute to identifying new therapeutic targets for epilepsy treatment.
The research emphasizes the importance of spatial and temporal analysis in understanding seizure activity.

Frequently Asked Questions

What are the advantages of using CMOS-HD-MEAs?

CMOS-HD-MEAs allow for high-resolution recordings of electrophysiological data, providing detailed insights into brain activity and seizure dynamics.

How is seizure activity recorded in this study?

Seizure activity is recorded from brain slices using CMOS-HD-MEAs, which capture local field potentials during various treatment paradigms.

What types of data can be obtained from the CMOS-HD-MEAs?

The technology enables the recording of high-resolution local field potentials, facilitating analysis of seizure patterns and dynamics in the brain.

How can the method be applied to other research?

This protocol can be adapted for studying various neurological disorders by altering the treatment paradigms applied to the brain slices.

Are there any limitations to using this approach?

Some limitations may include the variability between brain slices and the need for precise experimental conditions to ensure consistent results.

Hier skizzieren wir ein Protokoll für die Verwendung komplementärer Metalloxid-Halbleiter-Mikroelektroden-Array-Systeme mit hoher Dichte (CMOS-HD-MEAs) zur Aufzeichnung anfallsähnlicher Aktivität aus ex vivo Hirnschnitten.

Im Parrish-Labor sind wir daran interessiert zu verstehen, wie Anfälle beginnen, sich ausbreiten und enden. Wir sind besonders daran interessiert, neuartige Therapien für den Status epilepticus zu erforschen, eine lebensbedrohliche Erkrankung, bei der ein Anfall nicht von selbst endet. In unserer Forschung verwenden wir CMOS-Mikroelektrodenarray-Systeme mit hoher Dichte.

Diese fortschrittlichen Technologien ermöglichen es uns, hochauflösende elektrophysiologische Daten von Hirnschnitten aufzuzeichnen und detaillierte lokale Feldpotentiale zu erfassen. Dies hilft uns, komplexe Gehirnaktivitäten wie Anfallsmuster mit großer räumlicher und zeitlicher Präzision zu verstehen. In Zukunft planen wir, die räumlichen und zeitlichen Ausbreitungsmuster des Status epilepticus zu erforschen, einem verlängerten Anfallszustand, der oft resistent gegen antiepileptische Medikamente wird.

Wir planen, die Informationen aus diesen Studien zu nutzen, um wirksamere Behandlungen für den Status epilepticus zu finden.

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Neurowissenschaften Ausgabe 211 Neurowissenschaften Elektrophysiologie getauchte Kammer ex vivo Hippocampus Neokortex 4-Aminopyridin 0 Magnesium Nagetierhirnschnitte lokales Feldpotential iktal HD-MEA