Name: September 2013 - This Month in JoVE (Video) | JoVE Journal
Uploaded: 2013-09-03T09:00:00.000+00:00
Duration: 3 min 40 s
Description: Explore advanced methods in developmental biology, bioengineering, and chemistry featured in this issue. Learn live imaging of zebrafish craniofacial development, bioreactor cell culture systems, and dual-camera fluorescence techniques for studying cell adhesion and disease mechanisms.

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5 minutes

<h2 class="jove_title"><a href="https://www.jove.com/video/50525">Visualization of Craniofacial Development in the sox10: kaede Transgenic Zebrafish Line Using Time-lapse Confocal Microscopy</a></h2>
Lisa Gfrerer1, Max Dougherty1, Eric C. Liao1
1<a href='/institutions/NA-north-america/US-united-states/MA-massachusetts/24866-massachusetts-general-hospital'>Center for Regenerative Medicine, Massachusetts General Hospital</a>
Visualization of experimental data has become a key element in presenting results to the scientific community. Generation of live time-lapse recording of growing embryos contributes to better presentation and understanding of complex developmental processes. This protocol is a step-by-step guide to cell labeling via photoconversion of kaede protein in zebrafish.
<h2 class="jove_title"><a href="https://www.jove.com/video/50582">Operation of a Benchtop Bioreactor</a></h2>
Kristina M. Obom1, Andrew Magno2, Patrick J. Cummings1
1Center for Biotechnology Education, Krieger School of Arts and Sciences, Johns Hopkins University, 2ATR Biotech
Fermentors are used to increase culture yield and productivity of bioengineered cells. After screening multiple microbial or animal cell culture candidates in shake flasks, the next logical step is to increase the selected culture's biomass with the fermentor. This video demonstrates the setup and operation of a typical benchtop bioreactor system.
<h2 class="jove_title"><a href="https://www.jove.com/video/50604">Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion</a></h2>
Grady E. Carlson1, Eric W. Martin2, Monica M. Burdick1,2
1Department of Chemical and Biomolecular Engineering, Russ College of Engineering and Technology, Ohio University, 2Biomedical Engineering Program, Ohio University
Dual camera emission splitting systems for two-color fluorescence microscopy generate real-time image sequences with exceptional optical and temporal resolution, a requirement of certain live cell assays including parallel plate flow chamber adhesion assays. When software is employed to merge images from simultaneously acquired emission channels, pseudocolored image sequences are produced.
<h2 class="jove_title"><a href="https://www.jove.com/video/50757">Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry</a></h2>
Evan L. Pannkuk1, Thomas S. Risch2, Brett J. Savary3
1Graduate Program of Environmental Science, Arkansas State University, 2Department of Biological Sciences, Arkansas State University, 3Arkansas Biosciences Institute and College of Agriculture and Technology, Arkansas State University
Mammalian integument contains solvent-extractable lipids that can provide chemical compositions characteristic of individual species. This paper presents a routine method for separating broad lipid classes isolated from integumentary tissues using thin layer chromatography and determining the triacylglyceride profile by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

september 2013 - this month in jove

September 2013 - This Month in JoVE

Here's a look at what's coming up in the <a href="http://www.jove.com/archive/79">September 2013 issue</a> of <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.
Palatogenesis (palate formation) involves complex developmental processes that are highly regulated and conserved among vertebrates. Thus, scientists use laboratory models like the zebrafish to study cleft palate and related developmental malformations. <a href="http://www.jove.com/video/50525">Gfrerer et al.</a> examine zebrafish embryos using time-lapse confocal microscopy, which lets them observe the migration of cranial neural crest cells (CNCCs) from the dorsal neural tube to form craniofacial structures. This method also allows live imaging of abnormal craniofacial development in zebrafish, which is used to model conditions like cleft palate in humans.
In the laboratory, bacteria, yeast, and many other cell types are commonly grown in shake flasks. However, this classic technique has many drawbacks that can prevent optimal growth, such as fluctuations in pH, oxygen concentration, nutrients, and temperature. In <a href="http://www.jove.com/bioengineering">JoVE Bioengineering</a>, <a href="http://www.jove.com/video/50582">Obom et al.</a> demonstrate a benchtop bioreactor complete with a pH probe an oxygen probe, reagent feed bottle to replenish media, precise temperature controls, a gas infuser, and an impeller that stirs the culture. This system allows optimal culture conditions for the large-scale growth of recombinant expression systems.
Also in <a href="http://www.jove.com/bioengineering">JoVE Bioengineering</a>, we have a method for live cell imaging in a flow-based cell adhesion assay. But unlike standard protocols that use single camera systems, our featured method is a dual camera emission splitting system that captures real-time images in two fluorescence channels at the same time. <a href="http://www.jove.com/video/50604">Carlson et al.</a> use a parallel plate flow chamber adhesion assay to demonstrate this dual camera system, which they use to study adhesion, rolling, and other cell behaviors.
<a href="http://www.jove.com/chem">JoVE Chemistry</a> features a not-so-common animal model: the Eastern red bat, which is widespread in the United States; however, in recent years, a fungal disease called white nose syndrome (WNS) has killed millions of these bats. WNS is a major threat to the agricultural industry, which relies on bats as a form of natural pest control. Like other mammals, bats have sebaceous glands in their skin that secrete sebum, an oily material that helps protect against infections. <a href="http://www.jove.com/video/50757">Pannkuk et al.</a> demonstrate a method for isolating the sebum from the hair and the skin of the bat, then analyze the lipid composition of the sebum using thin-layer chromatography (TLC) combined with matrix-assisted laser desorption/ionization time-of-flight (MALTI-TOF) mass spectrometry. The lipid profile of the sebum can affect immune processes and can also serve as disease markers; thus, this method will yield vital information for disease treatment and prevention in the Eastern red bat as well as other mammals.
You&#8217;ve just had a sneak peek of a few highlights from the <a href="http://www.jove.com/archive/79">September 2013 issue</a> of JoVE. Visit the website to see the full-length articles, plus many more, in <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.

Here's a look at what's coming up in the <a href="http://www.jove.com/archive/79">September 2013 issue</a> of <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.

Here's a look at what's coming up in the September 2013 issue of JoVE: The Journal of Visualized Experiments.
Palatogenesis (palate formation) involves ...

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Explore advanced methods in developmental biology, bioengineering, and chemistry featured in this issue. Learn live imaging of zebrafish craniofacial development, bioreactor cell culture systems, and dual-camera fluorescence techniques for studying cell adhesion and disease mechanisms.

September 2013 - This Month in JoVE (Video) | JoVE Journal

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September 2013 - This Month in JoVE

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