Heli Skottman BioMediTech Institute, Faculty of Medicine and Life Sciences University of Tampere Biography Publications Institution JoVE Articles Heli Skottman has not added a biography. If you are Heli Skottman and would like to personalize this page please email our Author Liaison for assistance. Publications Small Non-coding RNA Landscape of Extracellular Vesicles from Human Stem Cells Scientific Reports. Oct, 2018 | Pubmed ID: 30341351 Loss of NRF-2 and PGC-1α Genes Leads to Retinal Pigment Epithelium Damage Resembling Dry Age-related Macular Degeneration Redox Biology. Sep, 2018 | Pubmed ID: 30253279 Wound Healing of Human Embryonic Stem Cell-derived Retinal Pigment Epithelial Cells is Affected by Maturation Stage Biomedical Engineering Online. Jul, 2018 | Pubmed ID: 30064430 Hsp90 Inhibition As a Means to Inhibit Activation of the NLRP3 Inflammasome Scientific Reports. Apr, 2018 | Pubmed ID: 29712950 Human Stem Cell Based Corneal Tissue Mimicking Structures Using Laser-assisted 3D Bioprinting and Functional Bioinks Biomaterials. Jul, 2018 | Pubmed ID: 29684677 Hydrazone Crosslinked Hyaluronan-based Hydrogels for Therapeutic Delivery of Adipose Stem Cells to Treat Corneal Defects Materials Science & Engineering. C, Materials for Biological Applications. Apr, 2018 | Pubmed ID: 29407158 Breath Figures in Tissue Engineering and Drug Delivery: State-of-the-art and Future Perspectives Acta Biomaterialia. 01, 2018 | Pubmed ID: 29183847 Xeno- and Feeder-free Differentiation of Human Pluripotent Stem Cells to Two Distinct Ocular Epithelial Cell Types Using Simple Modifications of One Method Stem Cell Research & Therapy. 12, 2017 | Pubmed ID: 29284513 Comparative Proteomic Analysis of Human Embryonic Stem Cell-derived and Primary Human Retinal Pigment Epithelium Scientific Reports. Jul, 2017 | Pubmed ID: 28729539 Autophagy Regulates Proteasome Inhibitor-Induced Pigmentation in Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells International Journal of Molecular Sciences. May, 2017 | Pubmed ID: 28534814 RNA Polymerase III Subunit POLR3G Regulates Specific Subsets of PolyA and SmallRNA Transcriptomes and Splicing in Human Pluripotent Stem Cells Stem Cell Reports. 05, 2017 | Pubmed ID: 28494942 Langmuir-Schaefer Film Deposition Onto Honeycomb Porous Films for Retinal Tissue Engineering Acta Biomaterialia. 05, 2017 | Pubmed ID: 28223209 Poly(trimethylene Carbonate) As an Elastic Biodegradable Film for Human Embryonic Stem Cell-derived Retinal Pigment Epithelial Cells Journal of Tissue Engineering and Regenerative Medicine. Nov, 2017 | Pubmed ID: 28052579 Semi-automatic Method for Ca Imaging Data Analysis of Maturing Human Embryonic Stem Cells-Derived Retinal Pigment Epithelium Annals of Biomedical Engineering. Nov, 2016 | Pubmed ID: 27225993 Honeycomb Porous Films As Permeable Scaffold Materials for Human Embryonic Stem Cell-derived Retinal Pigment Epithelium Journal of Biomedical Materials Research. Part A. 07, 2016 | Pubmed ID: 26914698 Texture Descriptors Ensembles Enable Image-Based Classification of Maturation of Human Stem Cell-Derived Retinal Pigmented Epithelium PloS One. 2016 | Pubmed ID: 26895509 Human Pluripotent Stem Cell-derived Limbal Epithelial Stem Cells on Bioengineered Matrices for Corneal Reconstruction Experimental Eye Research. 05, 2016 | Pubmed ID: 26658714 Ultrathin Polyimide Membrane As Cell Carrier for Subretinal Transplantation of Human Embryonic Stem Cell Derived Retinal Pigment Epithelium PloS One. 2015 | Pubmed ID: 26606532 Comparative Proteomics Reveals Human Pluripotent Stem Cell-derived Limbal Epithelial Stem Cells Are Similar to Native Ocular Surface Epithelial Cells Scientific Reports. Oct, 2015 | Pubmed ID: 26423138 Human IPSC Derived Disease Model of MERTK-associated Retinitis Pigmentosa Scientific Reports. Aug, 2015 | Pubmed ID: 26263531 Computational Model of Ca2+ Wave Propagation in Human Retinal Pigment Epithelial ARPE-19 Cells PloS One. 2015 | Pubmed ID: 26070134 Patient-Specific Induced Pluripotent Stem Cell-Derived RPE Cells: Understanding the Pathogenesis of Retinopathy in Long-Chain 3-Hydroxyacyl-CoA Dehydrogenase Deficiency Investigative Ophthalmology & Visual Science. May, 2015 | Pubmed ID: 26024122 Surface Modified Biodegradable Electrospun Membranes As a Carrier for Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells Tissue Engineering. Part A. Sep, 2015 | Pubmed ID: 25946229 Biomimetic Collagen I and IV Double Layer Langmuir-Schaefer Films As Microenvironment for Human Pluripotent Stem Cell Derived Retinal Pigment Epithelial Cells Biomaterials. May, 2015 | Pubmed ID: 25771016 Small-molecule Induction Promotes Corneal Epithelial Cell Differentiation from Human Induced Pluripotent Stem Cells Stem Cell Reports. Feb, 2014 | Pubmed ID: 24527395 Structure and Barrier Properties of Human Embryonic Stem Cell-derived Retinal Pigment Epithelial Cells Are Affected by Extracellular Matrix Protein Coating Tissue Engineering. Part A. Feb, 2014 | Pubmed ID: 24044751 Three-dimensional Growth Matrix for Human Embryonic Stem Cell-derived Neuronal Cells Journal of Tissue Engineering and Regenerative Medicine. Mar, 2014 | Pubmed ID: 22611014 Healthy Human CSF Promotes Glial Differentiation of HESC-derived Neural Cells While Retaining Spontaneous Activity in Existing Neuronal Networks Biology Open. Jun, 2013 | Pubmed ID: 23789111 Aquaporin Expression and Function in Human Pluripotent Stem Cell-derived Retinal Pigmented Epithelial Cells Investigative Ophthalmology & Visual Science. May, 2013 | Pubmed ID: 23687169 Effects of Different Serum Conditions on Osteogenic Differentiation of Human Adipose Stem Cells in Vitro Stem Cell Research & Therapy. Feb, 2013 | Pubmed ID: 23415114 Comparative Analysis of Targeted Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) and Human Embryonic Stem Cells Reveals Variability Associated with Incomplete Transgene Silencing in Retrovirally Derived HiPSC Lines Stem Cells Translational Medicine. Feb, 2013 | Pubmed ID: 23341440 Towards a Defined, Serum- and Feeder-free Culture of Stratified Human Oral Mucosal Epithelium for Ocular Surface Reconstruction Acta Ophthalmologica. Dec, 2013 | Pubmed ID: 22963401 The Effect of Human and Mouse Fibroblast Feeder Cells on Cardiac Differentiation of Human Pluripotent Stem Cells Stem Cells International. 2012 | Pubmed ID: 22315618 Efflux Protein Expression in Human Stem Cell-derived Retinal Pigment Epithelial Cells PloS One. 2012 | Pubmed ID: 22272278 Laminin-511 Expression is Associated with the Functionality of Feeder Cells in Human Embryonic Stem Cell Culture Stem Cell Research. Jan, 2012 | Pubmed ID: 22099024 A Xeno-free Culturing Protocol for Pluripotent Stem Cell-derived Oligodendrocyte Precursor Cell Production Regenerative Medicine. Jul, 2011 | Pubmed ID: 21749203 Toward the Defined and Xeno-free Differentiation of Functional Human Pluripotent Stem Cell-derived Retinal Pigment Epithelial Cells Molecular Vision. Feb, 2011 | Pubmed ID: 21364903 Human Cell-based Micro Electrode Array Platform for Studying Neurotoxicity Frontiers in Neuroengineering. 2010 | Pubmed ID: 20953240 Similarly Derived and Cultured HESC Lines Show Variation in Their Developmental Potential Towards Neuronal Cells in Long-term Culture Regenerative Medicine. Sep, 2010 | Pubmed ID: 20868330 Production and Isolation of NG2+ Oligodendrocyte Precursors from Human Embryonic Stem Cells in Defined Serum-free Medium Stem Cell Research. Sep, 2010 | Pubmed ID: 20538536 A Defined and Xeno-free Culture Method Enabling the Establishment of Clinical-grade Human Embryonic, Induced Pluripotent and Adipose Stem Cells PloS One. Apr, 2010 | Pubmed ID: 20419109 Human Embryonic Stem Cell-derived Cardiomyocytes: Demonstration of a Portion of Cardiac Cells with Fairly Mature Electrical Phenotype Experimental Biology and Medicine (Maywood, N.J.). Apr, 2010 | Pubmed ID: 20407085 Spatial and Temporal Expression Pattern of Germ Layer Markers During Human Embryonic Stem Cell Differentiation in Embryoid Bodies Histochemistry and Cell Biology. May, 2010 | Pubmed ID: 20369364 Derivation and Characterization of Three New Human Embryonic Stem Cell Lines in Finland In Vitro Cellular & Developmental Biology. Animal. Apr, 2010 | Pubmed ID: 20177999 Transcriptome Profiling of Human Pre-implantation Development PloS One. Nov, 2009 | Pubmed ID: 19924284 CD Marker Expression Profiles of Human Embryonic Stem Cells and Their Neural Derivatives, Determined Using Flow-cytometric Analysis, Reveal a Novel CD Marker for Exclusion of Pluripotent Stem Cells Stem Cell Research. Mar, 2009 | Pubmed ID: 19383417 Substantial Variation in the Cardiac Differentiation of Human Embryonic Stem Cell Lines Derived and Propagated Under the Same Conditions--a Comparison of Multiple Cell Lines Annals of Medicine. 2009 | Pubmed ID: 19165643 Comparison of Biomaterials and Extracellular Matrices As a Culture Platform for Multiple, Independently Derived Human Embryonic Stem Cell Lines Tissue Engineering. Part A. Jul, 2009 | Pubmed ID: 19132919 Challenges and Approaches to the Culture of Pluripotent Human Embryonic Stem Cells Regenerative Medicine. May, 2007 | Pubmed ID: 17511563 Monitoring and Analysis of Dynamic Growth of Human Embryonic Stem Cells: Comparison of Automated Instrumentation and Conventional Culturing Methods Biomedical Engineering Online. Apr, 2007 | Pubmed ID: 17428350 Testing of Nine Different Xeno-free Culture Media for Human Embryonic Stem Cell Cultures Human Reproduction (Oxford, England). May, 2007 | Pubmed ID: 17251355 Culture Conditions for Human Embryonic Stem Cells Reproduction (Cambridge, England). Nov, 2006 | Pubmed ID: 17071770 The Derivation of Clinical-grade Human Embryonic Stem Cell Lines FEBS Letters. May, 2006 | Pubmed ID: 16716780 Integrating Probe-level Expression Changes Across Generations of Affymetrix Arrays Nucleic Acids Research. Dec, 2005 | Pubmed ID: 16356924 Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells Heidi Hongisto*1, Meri Vattulainen*1, Tanja Ilmarinen1, Alexandra Mikhailova2, Heli Skottman1 1BioMediTech Institute, Faculty of Medicine and Life Sciences, University of Tampere, 2Department of Ophthalmology, SILK, Faculty of Medicine and Life Sciences, University of Tampere JoVE 58279 Biología del desarrollo
Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells Heidi Hongisto*1, Meri Vattulainen*1, Tanja Ilmarinen1, Alexandra Mikhailova2, Heli Skottman1 1BioMediTech Institute, Faculty of Medicine and Life Sciences, University of Tampere, 2Department of Ophthalmology, SILK, Faculty of Medicine and Life Sciences, University of Tampere JoVE 58279 Biología del desarrollo