Summary
The localization and distribution of proteins provide important information for understanding their cellular functions. The superior spatial resolution of electron microscopy (EM) can be used to determine the subcellular localization of a given antigen following immunohistochemistry. For tissues of the central nervous system (CNS), preserving structural integrity while maintaining antigenicity has been especially difficult in EM studies. Here, we adopt a procedure that has been used to preserve structures and antigens in the CNS to study and characterize synaptic proteins in rat hippocampal CA1 pyramidal neurons.
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Embed code vs URL
An embed code allows you to place a JoVE video directly inside of your learning or training environment. Most learning management systems such as Canvas, Blackboard, and Moodle require an embed code for JoVE content to stream.
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What is an Embed Code?
Basically, it’s a snippet of code that you put on your website that displays as a video. An embed code pulls the video from the original source, allowing you to display a video without having to host the file on your website.
How to embed a video
STEP 1: COPY YOUR EMBED CODE
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STEP 2: PASTE THE EMBED CODE INTO YOUR ENVIRONMENT
Embed codes can be used in most learning management systems (Canvas, Blackboard, Moodle…), organizational websites, and a host of other tools. The specifics of how to add in an embed code will depend on your environment.
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