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Studying DNA and RNA
Real-Time RT-PCR for Gene Expression Tracking
Description
Real-time RT-PCR tracks gene expression by measuring how much target DNA is made during PCR. It starts with mRNA, which is converted into complementary DNA, or cDNA, by an enzyme called reverse transcriptase. The cDNA is then amplified by PCR so the target sequence can be measured.
This method ca...
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Transcript
La reacción en cadena de la polimerasa con transcriptasa inversa en tiempo real, o RT-PCR en tiempo real, puede cuantificar un ARN de interés a medida que avanza la reacción.
Para empezar, la transcriptasa inversa copia el ARN en complementario, o ADNc. La ARNasa H digiere el ARN original, dejando pequeños cebadores unidos al ADNc.
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