This is a free preview. For full access
Studying DNA and RNA
Finding cDNA Ends with RACE PCR
Description
Rapid Amplification of cDNA Ends, or RACE, helps scientists capture the full-length cDNA from an mRNA sequence. It works by linking a known internal region to the unknown sequence at either the 5' end or the 3' end. The method is widely used when the missing end of a gene needs to be identified.
...
Show More
Transcript
Full-length cDNA is not always obtained when novel mRNA gene transcripts are first discovered. Unknown sequences at the ends of the mRNA template can result in partially cloned cDNAs.
The sequence of these partial cDNAs can be extended by a technique called Rapid Amplification of cDNA Ends or RACE to obtain a complete cDNA for the transcr...
Show More