Automated Deposition
Automated Deposition - Related Videos
11:30
A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
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Cited by 2 •
2022
Cardiovascular disease is the leading cause of death worldwide. Vascular calcification contributes substantially to the burden of cardiovascular morbidity and mortality. This protocol describes a simple method to quantify vascular smooth muscle cell-mediated calcium precipitation in vitro by fluorescent imaging.
02:33
Phase Transitions: Sublimation and Deposition
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2020
Some solids can transition directly into the gaseous state, bypassing the liquid state, via a process known as sublimation. At room temperature and standard pressure, a piece of dry ice (solid CO2) sublimes, appearing to gradually disappear without ever forming any liquid. Snow and ice sublimate at temperatures below the melting point of water, a slow process that may be accelerated by winds and the reduced atmospheric pressures at high altitudes. When solid iodine is warmed, the solid sublimes...
08:06
Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
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Cited by 1 •
2016
Peptide adsorption to plasticware during traditional tip-based serial dilutions can significantly impact potency determination and confound the understanding of structure-activity relationships used for lead identification and lead optimization phases of drug discovery. Here methods for automated acoustic non-contact serial dilution of peptide samples are described.
10:55
Digital Microfluidics for Automated Proteomic Processing
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Cited by 30 •
2009
Digital Microfluidics is a technique characterized by the manipulation of discrete droplets (~nL - mL) on an array of electrodes by the application of electrical fields. It is well-suited for carrying out rapid, sequential, miniaturized automated biochemical assays. Here, we report a platform capable of automating several proteomic processing steps.
10:52
Formation of Thick Dense Yttrium Iron Garnet Films Using Aerosol Deposition
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Cited by 7 •
2015
This report describes the use of a custom-built system to perform aerosol deposition of thick films of yttrium iron garnet onto sapphire substrates at RT. The deposited films are characterized using scanning electron microscopy, profilometry, and ferromagnetic resonance to give a representative overview of the capabilities of the technique.
FAQs
Programmed motion coordinates the deposition head with material flow so that a bioink, cell-containing material, polymer, or other biomaterial reaches the intended position. Spatial positioning determines where features appear, while flow and speed affect how much material is placed and how accurately patterns are reproduced. Coordinating these variables helps form structures without sacrificing properties needed for biological function.
Deposition speed and material flow are coupled process variables. Changing speed alters how quickly the system moves across a location, whereas changing flow alters the amount delivered through the nozzle or head. Spatial positioning adds geometric control. Together, these settings influence feature formation, pattern fidelity, and the ability to reproduce a programmed design consistently across fabricated structures.
Layer-by-layer construction converts a digital design into a three-dimensional arrangement of deposited material. Each successive layer contributes to the overall geometry, allowing the system to create organized features rather than relying on a single manual placement step. In bioengineering, this organization is important when fabricating scaffolds, tissue-engineering structures, or other models whose biological function depends on controlled form.
Compared with manual handling, automated deposition reduces variation caused by inconsistent placement and makes the fabrication sequence reproducible. Computer-controlled motion also allows researchers to repeat a programmed pattern, adjust defined process variables, and work at a scale that is difficult to maintain manually. These advantages improve experimental control when biological materials must be arranged in consistent spatial configurations.
A typical workflow starts with a digital design that specifies the intended pattern or structure. The system then uses programmed motion to guide a nozzle or deposition head while dispensing the selected bioink, cells, polymer, or other biomaterial. Repeated passes can build layers, and control of flow, speed, and position determines how closely the fabricated result follows the design.
Researchers apply automated deposition to tissue engineering, scaffold fabrication, organoid assembly, and engineered biological models. The same approach supports regenerative-medicine and biomedical studies by producing material arrangements with greater consistency and scalability than manual handling. Its value is not limited to shaping objects: controlled placement can also improve experimental comparisons by making fabrication conditions more uniform.