Canine Liver
Canine Liver - Related Videos
07:38
Real-time Monitoring of High Intensity Focused Ultrasound (HIFU) Ablation of In Vitro Canine Livers Using Harmonic Motion Imaging for Focused Ultrasound (HMIFU)
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Cited by 13 •
2015
This article describes real-time monitoring of HIFU ablation in canine liver with high frame rate ultrasound imaging using diverging and plane wave imaging. Harmonic Motion Imaging for Focused Ultrasound is used to image the decrease of acoustic radiation force induced displacement in the ablated region.
09:34
In Vivo Canine Muscle Function Assay
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Cited by 19 •
2011
We describe a minimally-invasive and painless method to measure canine hindlimb muscle strength and muscle response to repeated eccentric contractions.
09:37
Isolation and Expansion of Adult Canine Hippocampal Neural Precursors
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Cited by 3 •
2016
The canine brain is a valuable model in which to study adult neurogenesis. Presented here are protocols for isolating and expanding adult canine hippocampal neural precursor cells from primary brain tissue.
08:24
Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins
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Cited by 11 •
2016
Novel isolation methods of primary endothelial cells from blood vessels are needed. This protocol describes a new technique that completely inverts blood vessels of interest, exposing only the endothelial side to enzymatic digestion. The resulting pure endothelial cell culture can be used to study cardiovascular diseases, disease modelling, and angiogenesis.
06:45
A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release
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Cited by 8 •
2016
Neutrophil extracellular traps (NETs) are networks of DNA, histones and neutrophil proteins. Although a component of the innate immune response, NETs are implicated in autoimmunity and thrombosis. This protocol describes a simple method for canine neutrophil isolation and quantification of NETs using a microplate fluorescence assay.
FAQs
Hepatocytes provide the cellular basis for modeling liver metabolism and detoxification because they process nutrients and foreign compounds through enzyme-mediated biochemical reactions. A useful engineered system therefore needs to preserve or reproduce these functional activities rather than represent liver tissue only as a structural material. Measuring such reactions can help determine whether an organoid, scaffold, or perfused construct captures relevant liver behavior.
The specialized vascular network delivers blood and supports exchange between sinusoids and liver cells. Bioengineered constructs must therefore account for both circulation and cell access to the surrounding vascular environment when attempting to reproduce liver function. Perfused tissue models are especially relevant because their design directly addresses the role of blood delivery and exchange in maintaining organized liver-like activity.
Bile production connects liver activity with digestion, so it represents a functional feature beyond nutrient processing and detoxification. When researchers evaluate engineered canine liver systems, bile-related function can provide another indication of whether the model reproduces important organ behavior. This consideration broadens assessment from isolated cellular reactions to coordinated functions relevant to the liver’s physiological role.
Models should aim to capture the interaction between hepatocyte activity, vascular organization, sinusoid-mediated exchange, and bile-related function. Depending on the research goal, this can involve organoids, biomaterial scaffolds, or perfused tissue models. Reproducing these linked features is important because a system that preserves only one component may not reflect the combined structural and functional behavior of the canine liver.
These approaches provide complementary ways to reproduce key aspects of canine liver structure and function. Organoids can serve as organized model systems, biomaterial scaffolds can provide engineered structural support, and perfused tissue models can address blood delivery and exchange. The appropriate format depends on whether the study emphasizes tissue organization, engineered architecture, or vascularly supported function.
Engineered systems can support studies of liver disease, drug evaluation, and regenerative or replacement therapies. Their value comes from providing models that reproduce selected aspects of organ structure and function without relying solely on an intact liver. Researchers can select organoids, scaffolds, or perfused constructs according to the biological question and the functions they need to examine.