Immunocytochemical Studies
Immunocytochemical Studies - Related Videos
09:56
Small-scale Propagation of Human iPSCs in Serum-free Conditions for Routine Immunocytochemical Characterization
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2017
Regular characterization of induced pluripotent stem cells (iPSCs), to ascertain maintenance of their pluripotent state, is an important step before these cells are used for other applications. Here we describe a method for the small-scale propagation of human iPSCs specifically designed to enable their easy and routine characterization via immunocytochemistry.
09:16
Immunocytochemical Analysis of Human Pluripotent Stem Cells using a Self-Made Cytospin Apparatus
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Cited by 1 •
2010
Suspension immunocytochemical staining of human pluripotent stem cells (hPSCs) for cell-surface markers (SSEA-3/SSEA-4) was achieved based on use of a self-made cytospin apparatus to create a monolayer of cells for observation and quantification.
10:27
Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis
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Cited by 2 •
2018
This article details the methods that are used to expand human fetal brain neural stem cells in culture, as well as how to differentiate them into various neuronal subtypes and astrocytes, with an emphasis on the use of neural stem cells to study Zika virus infection.
11:22
Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle
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Cited by 39 •
2015
The main adherent cell types derived from human muscle are myogenic cells and fibroblasts. Here, cell populations are enriched using magnetic-activated cell sorting based on the CD56 antigen. Subsequent immunolabelling with specific antibodies and use of image analysis techniques allows quantification of cytoplasmic and nuclear characteristics in individual cells.
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Case Studies
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2020
There are many research methods available to psychologists in their efforts to understand, describe, and explain behavior and the cognitive and biological processes that underlie it. In 2011, the New York Times published a feature story on Krista and Tatiana Hogan, Canadian twin girls. These particular twins are unique because Krista and Tatiana are conjoined twins, connected at the head. There is evidence that the two girls are connected in a part of the brain called the thalamus, which is a...
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The primary antibody provides target selectivity by binding the chosen antigen, while the labeled secondary antibody converts that binding event into a fluorescent or enzymatic signal. Microscopy then reveals where the target occurs within an individual cell. This molecular-to-spatial connection helps relate marker distribution to cellular structure and function.
In the typical workflow, cells are fixed and permeabilized before antibody exposure. These preparation stages establish the conditions for applying the primary antibody and subsequent labeled secondary antibody. The sequence allows marker labeling to be assessed through microscopy in relation to cellular morphology, location, and organization.
Spatial localization lets investigators interpret a molecular marker together with the cell’s morphology and position. A signal can therefore indicate not only that a protein or other antigen is associated with a cell, but also where it appears within that cellular context. In bioengineering, this linkage supports evaluation of cell identity, differentiation, and signaling in engineered systems.
Fluorescent and enzymatic readouts provide alternative ways to detect antibody labeling by microscopy. A labeled secondary antibody generates one of these signal types after the primary antibody binds the target antigen. The selected readout changes the form in which the result is observed, while the underlying purpose remains localization of the selected marker within individual cells.
Within bioengineering, these studies can examine cell identity, differentiation, and signaling, as well as cellular responses to biomaterials or engineered microenvironments. The method connects these biological readouts with cellular location and morphology. That combination is useful when researchers need to assess how an engineered setting influences cells with spatial context.
They provide marker-based evidence for cells and engineered cell systems used in tissue engineering and regenerative medicine. By visualizing selected antigens in individual cells, investigators can relate molecular markers to morphology and location during assessment. The same approach also contributes to disease modeling, where cellular identity, differentiation, signaling, or responses to an engineered microenvironment are relevant outcomes.