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Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle
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Chapters
- 00:05Title
- 01:37Isolating Muscle-derived Precursor Cells
- 03:48Immunomagnetic Bead Sorting of CD56+ Cells
- 06:17Image Processing and Analysis of Fluorescently Labeled Nuclear Transcription Factors
- 08:58Results: Representative Characterization of Primary Human Skeletal Muscle Myogenic and Fibroblast Cells
- 10:43Conclusion
The main adherent cell types derived from human muscle are myogenic cells and fibroblasts. Here, cell populations are enriched using magnetic-activated cell sorting based on the CD56 antigen. Subsequent immunolabelling with specific antibodies and use of image analysis techniques allows quantification of cytoplasmic and nuclear characteristics in individual cells.
