Metabolic Assessment
Metabolic Assessment - Related Videos
07:54
Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy
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Cited by 6 •
2011
A progressive colonization procedure is described to further assess its impact on the host hepatic metabolism. Colonization is monitored non invasively by evaluating the urinary excretion of microbial co-metabolites by NMR-based metabolic profiling while hepatic metabolism is assessed by High Resolution Magic Angle Spinning (HR MAS) NMR profiling of intact biopsy.
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
00:52
What is Metabolism?
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2019
Overview Metabolism represents all of the chemical activity in a cell, including reactions that build molecules (anabolism) and those that break molecules down (catabolism). Anabolic reactions require energy, whereas catabolic reactions provide it. Thus, metabolism describes how cells transform energy through a variety of chemical reactions, which are often made more efficient with the help of enzymes. Metabolism Is the Sum of All of the Chemical Reactions That Take Place in an Organism
06:15
Assessing the Metabolic Activity of Whole Regenerating Zebrafish Ventricles Ex Vivo Using an Extracellular Flux Assay
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2026
In this article, we describe a detailed protocol for quantifying the oxygen consumption rate and extracellular acidification rate of ex vivo adult zebrafish ventricles to characterize their oxidative and glycolytic metabolic capacity.
FAQs
Oxygen consumption and carbon dioxide production provide different indicators of energy-related activity, while nutrient uptake and metabolite concentrations add information about material use and biochemical outputs. Examining these measures together can show how cells or engineered systems acquire and transform resources under defined conditions. This broader profile helps relate metabolic activity to growth, productivity, viability, and other performance outcomes.
Defined conditions make metabolic measurements interpretable by linking changes to specific factors such as genetic modifications, biomaterials, culture environments, or bioprocess parameters. Without a controlled comparison, shifts in oxygen use, nutrient uptake, or metabolite levels cannot be readily attributed to the factor being studied. Consistent conditions therefore support meaningful evaluation of cellular function and system performance.
Changes in metabolite concentrations can indicate that a system is processing nutrients differently or that energy-related pathways have been altered. When interpreted alongside oxygen consumption, carbon dioxide production, or nutrient uptake, these changes help connect biochemical activity with cellular behavior. In bioengineering, that connection can clarify how an engineered system responds to its design or culture environment.
A basic workflow begins by selecting indicators suited to the biological system and research question, such as gas exchange, nutrient uptake, metabolite concentrations, or pathway-related changes. Researchers then examine those indicators under defined conditions and compare the resulting measurements across the systems or parameters of interest. The final interpretation links metabolic changes with growth, productivity, viability, or therapeutic potential.
In bioreactor research, metabolic measurements help evaluate how bioprocess parameters influence cellular activity and overall system performance. Tracking oxygen consumption, carbon dioxide production, nutrient uptake, or metabolite concentrations can reveal whether operating conditions support desirable growth or productivity. These results provide a basis for comparing conditions and selecting settings that better maintain viability or improve engineered-system output.
For tissue engineering and engineered cells, metabolic assessment provides evidence of how cells function within a designed biological context. Measurements can show responses to biomaterials, culture environments, or genetic modifications and can be related to viability, growth, productivity, or therapeutic potential. The approach also supports disease modeling and drug development by connecting biochemical activity with the behavior of the tested system.