Nanofiber Scaffolds
Nanofiber Scaffolds - Related Videos
09:32
Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
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Cited by 5 •
2015
Here, we present a protocol to fabricate electrospun nanofiber scaffolds with gradated organization of fibers and explore their applications in regulating cell morphology/orientation. Gradients with regard to physical and chemical properties of the nanofiber scaffolds offer a wide variety of applications in the biomedical field.
06:14
Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds
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Cited by 6 •
2019
This article demonstrates the technique of expanding a traditional, two-dimension (2D) electrospun nanofiber mat into a three-dimension (3D) scaffold through the depressurization of subcritical CO2 fluid. These augmented scaffolds are 3D, closely mimic cellular nanotopographic cues, and preserve the functions of biologic molecules encapsulated within the nanofibers.
09:39
Procedure for Fabricating Biofunctional Nanofibers
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Cited by 1 •
2012
An efficient approach for preparing nanofibers decorated with functional groups capable of specifically interacting with proteins is described. The approach first requires the preparation of a polymer functionalized with the appropriate functional group. The functional polymer is fabricated into nanofibers by electrospinning. The effectiveness of the binding of the nanofibers with a protein is studied by confocal microscopy.
16:03
The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds
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Cited by 33 •
2011
Aligned electrospun fibers direct the growth of neurons in vitro and are a potential component of nerve regeneration scaffolds. We describe a procedure for preparing electrospun fiber substrates and the serum-free culture of primary rat E15 sensory (DRG) and motor neurons. Visualization of neurons by immunocytochemistry is also included.
14:43
Synthesis of Keratin-based Nanofiber for Biomedical Engineering
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Cited by 12 •
2016
Electrospun nanofibers have a high surface area to weight ratio, excellent mechanical integrity, and support cell growth and proliferation. These nanofibers have a wide range of biomedical applications. Here we fabricate keratin/ PCL nanofibers, using the electrospinning technique, and characterize the fibers for possible applications in tissue engineering.
FAQs
Fiber dimensions create a high surface-area-to-volume ratio, providing extensive sites for cell attachment. The interconnected spaces between fibers also support movement of nutrients, oxygen, and signaling molecules through the scaffold. Together, these structural features can affect how cells attach, organize, and contribute to tissue formation, making nanoscale architecture an important design variable in bioengineering.
Fiber alignment, diameter, composition, surface area, and porosity are key adjustable properties. Alignment and diameter can influence cell organization, while composition contributes biochemical cues that affect cell behavior. Porosity and interconnection influence transport through the material. Researchers therefore adjust these features together rather than treating scaffold structure and chemistry as separate design considerations.
Fiber alignment can provide an organized physical environment that influences cell arrangement and behavior. Because researchers can tune alignment during fabrication, they can design scaffolds with structural cues suited to particular tissue-engineering goals. This control helps move beyond simply supporting cell attachment by using the scaffold architecture to guide organization during tissue formation.
Researchers modify fiber alignment, diameter, composition, and porosity to match the intended application. These changes can alter attachment sites, transport of nutrients and signaling molecules, and the biochemical cues presented to cells. Fabrication approaches such as electrospinning provide a way to tune these characteristics, allowing scaffold designs to address different tissue and therapeutic requirements.
Electrospinning is one fabrication method used to produce and adjust nanofiber scaffold characteristics. In this context, researchers use the approach to control features such as fiber diameter, alignment, and composition. The resulting structure can then be selected or modified according to the desired cellular and transport environment, supporting application-specific material development.
Bioengineers use these scaffolds when they need a material that supports cell growth, organization, and tissue formation while reproducing selected aspects of the extracellular matrix. Their applications include tissue engineering, regenerative medicine, and drug delivery. The ability to adjust physical and biochemical properties makes them useful across projects with different cellular and therapeutic objectives.
Studies can show how changes in fiber structure, porosity, and composition affect cell attachment, organization, and tissue development. They can also evaluate how effectively nutrients, oxygen, and signaling molecules move through the material. These observations help researchers connect scaffold design choices with cellular outcomes and refine materials for regenerative or drug-delivery applications.