Lentiviral Gene Transfer
Lentiviral Gene Transfer - Related Videos
03:31
Laser-Assisted Lentiviral Gene Delivery: A Technique to Permeabilize Mouse Fertilized Eggs to Facilitate Lentiviral Gene Delivery
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2025
In this video, we demonstrate the laser-assisted permeation of the protective layer of zona pellucida in mouse fertilized eggs for facilitating lentiviral gene delivery. Lentivirus enables the generation of transgenic animals with a gene of interest stably integrated into their genome.
02:30
Gene Delivery to Mouse Intestinal Organoids Using Lentiviral Vectors
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2026
Source: Van Lidth de Jeude, J. F., et al. Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids. J. Vis. Exp. (2015)This video describes the lentiviral transduction of mouse intestinal organoids from a crypt-like structure containing suspension. Using a basement membrane matrix as a scaffold and growth factor–supplemented media, lentiviral vectors are introduced to enable stable gene...
01:27
Horizontal Gene Transfer
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2025
Horizontal gene transfer (HGT) is a process where genetic material moves between organisms within the same generation, unlike vertical gene transfer, which occurs from parent to offspring. HGT plays a crucial role in microbial evolution, adaptation, and survival, particularly in shared environments like the human gut.Mobile genetic elements such as plasmids, prophages, integrons, insertion sequences, and transposons facilitate this process. HGT occurs through three primary mechanisms:...
10:42
A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
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Cited by 21 •
2017
We describe the production strategy of integrase-deficient lentiviral vectors (IDLVs) as vehicles for delivering CRISPR/Cas9 to cells. With an ability to mediate quick and robust gene editing in cells, IDLVs present a safer and equally effective vector platform for gene delivery compared to integrase-competent vectors.
03:36
Production of Retroviral Particles for Gene Transfer
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2026
Source: Zjablovskaja, P., et al., Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells. J. Vis. Exp. (2018)This video demonstrates the production of recombinant retroviral particles by transfecting human kidney-derived packaging cells, enabling stable gene delivery to target cells for downstream applications such as genetic modification, functional assays, or lineage differentiation studies.
FAQs
After a lentiviral vector enters a target cell, its RNA genome is copied into DNA by reverse transcriptase. That DNA can integrate into the host genome, allowing the transferred sequence to remain associated with the cell as it persists and, when the cell divides, supports sustained gene expression.
Vector components can be modified to improve safety, reduce replication capacity, and control transgene expression. These changes let researchers tailor Lentiviral Gene Transfer to experimental needs while addressing the requirements of studies that depend on sustained expression or investigate therapeutic strategies for inherited and acquired disorders.
A key feature is compatibility with both dividing and nondividing target cells. This broadens the range of biological systems that can be investigated because the method is not limited to populations actively undergoing cell division. Researchers can therefore examine gene function or model disease in cellular settings where proliferation status matters experimentally.
At a conceptual level, experiments select a target cell population, expose it to a lentivirus-based vector carrying the genetic material of interest, and then study gene function or expression. The vector’s intracellular RNA-to-DNA conversion and genomic integration are central checkpoints because they connect genetic delivery with longer-term experimental effects.
Stable cell-line generation is one practical outcome of sustained genomic association. The same approach supports functional genomics, where researchers investigate gene function, and disease modeling, where engineered cells help represent biological features relevant to disorders. These applications make the technique useful for experiments requiring a persistent genetic change rather than a short-lived introduction.
In therapeutic research, the technique provides a platform for exploring strategies for both inherited and acquired disorders. Its relevance lies in combining genetic delivery with the possibility of sustained expression, while vector engineering offers ways to address safety, replication capacity, and expression control. These considerations guide research on cell and gene therapy concepts rather than defining a single treatment.