Biotinylated Oligonucleotides
Biotinylated Oligonucleotides - Related Videos
09:13
Cell-surface Biotinylation Assay
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2023
A cell can regulate the amount of particular proteins on its cell membrane through endocytosis, following which cell surface proteins are effectively sequestered in the cytoplasm. Once within a cell, these surface proteins can be either destroyed or “recycled” back to the membrane. The cell surface biotinylation assay provides researchers with a way to study these phenomena. The technique makes use of a derivative of the small molecule biotin, which can label surface proteins and then be...
11:32
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
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Cited by 20 •
2017
Here, we detail the method of Sequencing of Psoralen crosslinked, Ligated, and Selected Hybrids (SPLASH), which enables genome-wide mapping of intramolecular and intermolecular RNA-RNA interactions in vivo. SPLASH can be applied to study RNA interactomes of organisms including yeast, bacteria and humans.
06:43
In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia
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Cited by 1 •
2025
The protocol presents a method for in vivo covalent attachment of biotin to proteins based on their proximity to a biotin ligase fused to a protein of interest. This modification allows for a selective enrichment of the proteins using streptavidin beads as needed in protein interaction studies.
11:33
Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
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Cited by 86 •
2009
Microinjection is a well-established and effective method for introducing foreign substances into fertilized zebrafish embryos. Here, we demonstrate a robust microinjection technique for performing mRNA overexpression, and morpholino oligonucleotide gene knockdown studies in zebrafish.
12:03
Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells
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Cited by 3 •
2017
A modified density centrifugation gradient-based methodology was utilized to isolate epithelial cells from Rhipicephalus microplus gut tissue. Surface-bound proteins were biotinylated and purified through streptavidin magnetic beads for utilization in downstream applications.
FAQs
The biotin group provides an attachment point for streptavidin or avidin, which can be associated with a coated surface, magnetic bead, or other support. Binding the modified strand to that protein-containing support immobilizes it without removing its sequence-specific ability to interact with complementary DNA or RNA. This links molecular recognition to physical handling in an experiment.
The nucleotide sequence supplies programmable recognition because it can interact with a complementary nucleic acid sequence. Biotin contributes a separate handling function by enabling protein-mediated attachment to a support. Together, these features allow an oligonucleotide to identify a particular nucleic acid interaction while also being positioned for capture, detection, or isolation.
The support determines where the biotin-mediated attachment takes place. Coated surfaces can hold oligonucleotides in hybridization or detection formats, while magnetic beads and other supports provide alternative settings for affinity capture and targeted isolation. The underlying biotin–protein interaction remains the same, but the physical format can be selected to match the experimental workflow.
A typical workflow uses the oligonucleotide's sequence to establish a complementary interaction with a target DNA or RNA molecule, then uses the biotin group to attach that interaction to streptavidin- or avidin-associated support. The resulting immobilized complex can be used for purification, detection, or isolation of the selected nucleic acid or nucleic acid complex.
Supported applications include nucleic acid purification, hybridization assays, molecular detection, affinity capture, and targeted isolation of DNA or RNA complexes. These uses rely on the same combination of complementary-sequence recognition and biotin-mediated attachment, allowing researchers to adapt one molecular design to different analytical and separation-oriented tasks.
They provide a practical connection between programmable nucleotide recognition and protein-mediated separation. In genomics, diagnostics, and molecular biology, this connection supports experiments that need selected DNA or RNA sequences, hybridization-based readouts, or isolation of nucleic acid complexes. Their value lies in combining sequence-directed specificity with convenient immobilization and detection options.