Bundle Of His
Bundle Of His - Related Videos
08:50
Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
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Cited by 4 •
2013
We describe a procedure for profiling salivary proteins using multiplexed microsphere-based antibody arrays. Monoclonal antibodies were covalently linked to fluorescent dye-encoded 4.5 μm polymer microspheres using carbodiimide chemistry. The modified microspheres were deposited in fiber-optic microwells to measure protein levels in saliva using fluorescence sandwich immunoassays.
07:47
Directly Measuring Forces Within Reconstituted Active Microtubule Bundles
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Cited by 1 •
2022
Here, we present a protocol for reconstituting microtubule bundles in vitro and directly quantifying the forces exerted within them using simultaneous optical trapping and total internal reflection fluorescence microscopy. This assay allows for nanoscale-level measurement of the forces and displacements generated by protein ensembles within active microtubule networks.
06:08
Preparation and Immunofluorescence Staining of Bundles and Single Fiber Cells from the Cortex and Nucleus of the Eye Lens
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Cited by 7 •
2023
This protocol describes methods to prepare peripheral, mature, and nuclear eye lens fiber cells for immunofluorescence staining to study complex cell-to-cell interdigitations and the membrane architecture.
06:53
Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers
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Cited by 4 •
2022
Formation of actomyosin bundles in vitro and measuring myosin ensemble force generation using optical tweezers is presented and discussed.
06:47
Stereocilia Bundle Imaging with Nanoscale Resolution in Live Mammalian Auditory Hair Cells
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Cited by 2 •
2021
Here we present a protocol for the Hopping Probe Ion Conductance Microscopy (HPICM), a non-contact scanning probe technique that allows nanoscale imaging of stereocilia bundles in live auditory hair cells.
FAQs
The brief AV-node delay establishes the timing for ventricular activation after the impulse reaches the atrioventricular junction. This pause helps the signal proceed through the ventricular conduction pathway in an organized sequence rather than activating the ventricles prematurely. The resulting timing supports synchronized ventricular contraction and contributes to efficient blood pumping.
The Bundle of His provides the electrical route across the heart’s insulating fibrous skeleton, connecting the AV node with the ventricular conduction system. This passage is important because the impulse must move from the atrioventricular region into the ventricles before it can divide into the right and left bundle branches and continue toward ventricular muscle.
The right and left bundle branches divide the incoming signal into pathways directed toward the respective sides of the ventricles. They then distribute the impulse to Purkinje fibers and the ventricular myocardium. This branching arrangement helps coordinate activation across ventricular tissue, allowing contraction to occur in a synchronized pattern that supports effective cardiac pumping.
Electrocardiography is one way the functional importance of this conduction pathway becomes clinically useful. Because disruption can produce atrioventricular or bundle branch block, electrical recordings can contribute to recognizing abnormal cardiac conduction. The Bundle of His therefore connects cardiac physiology with clinical diagnosis, especially when interpreting findings related to ventricular activation and heartbeat coordination.
Disruption within the pathway can interfere with the coordinated delivery of electrical impulses to the ventricles. The overview identifies atrioventricular block and bundle branch block as possible consequences. These disturbances matter because impaired signal transmission can affect synchronized ventricular contraction, making the pathway important when assessing abnormalities in cardiac rhythm and pumping coordination.
Its study links the structure of specialized cardiac muscle cells with the timing and coordination of the heartbeat. Following the impulse from the AV node through the Bundle of His, its branches, Purkinje fibers, and ventricular myocardium shows how organized conduction supports efficient blood pumping. This makes the pathway relevant to physiology, electrocardiography, and clinical diagnosis.