Femoral Vein Sampling
Femoral Vein Sampling - Related Videos
10:45
Central Venous Catheter Insertion: Femoral Vein
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2023
Source: James W Bonz, MD, Emergency Medicine, Yale School of Medicine, New Haven, Connecticut, USA Central venous access is necessary in a multitude of clinical situations, including vascular access, vasopressor and caustic medication delivery, central venous pressure monitoring, volume resuscitation, total parental nutrition, hemodialysis, and frequent phlebotomy. There are three veins in the body that are accessed for central venous cannulation: the internal jugular, the subclavian, and the...
07:11
Sampling Blood from the Lateral Tail Vein of the Rat
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Cited by 82 •
2015
Blood samples are useful for assessing biomarkers of physiological states or disease in vivo. Here we describe the methodology to sample blood from the lateral tail vein in the rat. This method provides rapid samples with minimal pain and invasiveness.
09:38
Femoral Arterial and Venous Catheterization for Blood Sampling, Drug Administration and Conscious Blood Pressure and Heart Rate Measurements
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Cited by 53 •
2012
Chronic catheterization of blood vessels in the rat is often required for administration of substances, obtain blood sample over a period of time or for direct conscious blood pressure measurements. Femoral arterial catheterization of the rat and corresponding measurements of blood pressure in the conscious animal will be demonstrated.
03:52
Subclavian Vein Blood Sampling in Conscious Rats
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2023
Here we present a combination of effective rat restriction and subclavian vein puncture methods that enable rapid, safe, and repeated blood collection in rats without anesthesia.
07:10
Femoral Bone Marrow Aspiration in Live Mice
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Cited by 18 •
2014
This protocol describes a procedure for serial sampling of femoral bone marrow (BM) without requiring the sacrifice of mice. This procedure facilitates longitudinal studies of the BM composition of mice over time and provides serial access to cells within the BM for ex vivo and transplantation studies.
FAQs
Anatomical landmarks help identify the femoral vein’s expected position beneath the skin and provide a reference for directing the needle or catheter. This guidance supports entry into the intended vessel rather than surrounding tissue. In biological studies, consistent landmark-based positioning can help standardize collection across animals or clinical participants and improve the comparability of samples.
Negative pressure creates the force that moves venous blood through the needle or catheter and into the collection device. Without this pressure difference, blood would not be drawn efficiently into the container. The resulting sample can then be used to measure circulating cells, hormones, metabolites, antibodies, or drug concentrations, depending on the study objective.
Sterile technique helps limit contamination introduced during access to the vessel and reduces procedure-related complications. This is important because contamination can compromise biological measurements, while complications can affect the subject and interfere with later observations. Maintaining sterility therefore supports both sample integrity and reliable monitoring in animal research and clinical studies.
A basic workflow uses anatomical landmarks to locate the vessel, applies sterile technique, and guides a needle or catheter through the skin into the femoral vein. Negative pressure then draws blood into a collection device. The collected specimen can be directed toward the biological measurement required by the study, such as hormone or drug-concentration analysis.
Samples obtained through this approach can support measurements of circulating cells, hormones, metabolites, antibodies, and drug concentrations. The selected measurement depends on the biological question and the analysis planned after collection. This range allows investigators to examine cellular, biochemical, immune, and pharmacological features using blood obtained from the same general sampling approach.
The method is useful when investigators need blood-based information to monitor physiology, evaluate disease processes, or assess responses to treatment. Repeated sampling over time can help track changes during an experiment or study period. In animal research and clinical studies, these measurements connect circulating biological signals with disease status, physiological changes, or treatment effects.