Growth Chamber
Growth Chamber - Related Videos
03:50
Microscopic Analysis of Bacterial Growth Under Stress Using Microfluidic Chambers
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2025
Source: Cayron, J., et al. Multi-scale Analysis of Bacterial Growth Under Stress Treatments. J. Vis. Exp. (2019).This video demonstrates how to prepare and use a microfluidic plate for imaging bacterial cells under stress conditions. It outlines the process of introducing a bacterial culture and a stress-inducing antibiotic medium, followed by cell loading and microscopic setup. Time-lapse imaging in phase-contrast mode is then used to observe the development of elongated, filamentous cells...
09:02
Building and Operating a Low-Cost Elevated Carbon Dioxide Growth Chamber to Evaluate Microgreen Physiology under Spaceflight-Relevant CO2 Levels
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2026
This protocol describes how to construct and operate a small, low-cost growth chamber capable of maintaining extreme carbon dioxide (exCO2) levels for plant experiments. The chamber enables reproducible studies of microgreen physiology under spaceflight-relevant atmospheric conditions, using a single-board computer-based monitoring and control system and a passive hydroponic wicking system.
11:46
Axon Stretch Growth: The Mechanotransduction of Neuronal Growth
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Cited by 30 •
2011
A unique tissue engineering method was developed to elongate numerous nerve fibers in culture by recapitulating axon stretch growth; a form of nervous system growth whereby nerves elongate in conjunction with growth of the enlarging body.
07:33
A Customizable Chamber for Measuring Cell Migration
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Cited by 2 •
2017
This protocol details a customizable method to measure cell migration in response to chemoattractants that may also be used to determine the diffusion rate of a drug out of a polymer matrix.
10:45
In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures
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Cited by 4 •
2021
This protocol demonstrates a straightforward and robust method to study in situ axon growth and growth cone dynamics. It describes how to prepare ex vivo physiologically relevant acute brain slices and provides a user-friendly analysis pipeline.
FAQs
Growth Chamber experiments depend on isolating environmental variables and changing them deliberately. Researchers can set temperature, light intensity, light duration, humidity, and, when available, carbon dioxide concentration, then use sensors and programmable controls to maintain those settings. This design makes comparisons more interpretable because observed differences can be linked more directly to selected environmental conditions rather than uncontrolled fluctuations.
These components help the chamber remain near the selected conditions rather than merely receiving a one-time adjustment. Sensors detect environmental states, programmable controls adjust settings, and monitoring reveals whether conditions remain stable. Ventilation supports management of the enclosed environment. Together, they improve repeatability, which is essential when researchers compare biological responses across treatments or experimental runs.
Regulating light intensity and duration allows researchers to examine how plants or other biological materials respond to different illumination patterns. A programmable schedule can also support studies of circadian rhythms by making timing a deliberate experimental variable. Because other chamber conditions can be held at selected levels and monitored, investigators can compare responses to light conditions with greater consistency than in fluctuating natural settings.
They should select the environmental conditions relevant to the question, including temperature, light intensity and duration, humidity, and, where supported, carbon dioxide concentration. The chamber is then programmed and monitored so those settings remain consistent while the biological material grows. Researchers can compare resulting growth or responses among treatments, using the controlled setup to relate outcomes to specific environmental factors.
These chambers support investigations of plant development, photosynthesis, stress responses, circadian rhythms, and host-pathogen interactions. Their value differs by question: controlled light and temperature can support development or photosynthesis studies, while deliberately varied environmental conditions can reveal stress responses. The same controlled framework also helps examine timing-related biology and interactions between hosts and pathogens under repeatable conditions.
By reducing environmental fluctuations found in natural or greenhouse settings, the chamber strengthens controlled comparisons. If biological materials show different growth or responses under programmed conditions, researchers have a clearer basis for associating those outcomes with the environmental factor being tested. This consistency can improve experimental interpretation, while the findings remain tied to the selected chamber conditions.