P1 Adhesin
P1 Adhesin - Related Videos
10:05
Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches
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Cited by 15 •
2020
In this article, we describe, in detail, a protocol for the generation of neurosphere cultures from postnatal mouse neural stem cells derived from the main mouse neurogenic niches. Neurospheres are used to identify neural stem cells from brain tissue allowing the estimation of precursor cell numbers. Moreover, these 3D structures can be plated in differentiative conditions, giving rise to neurons, oligodendrocytes and astrocytes, allowing the study of cell fate.
08:13
Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes
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Cited by 17 •
2018
Here we present a protocol for the use of pre-existing antibiotic resistance-cassette deletion constructs as a basis for making deletion mutants in other E. coli strains. Such deletion mutations can be mobilized and inserted into the corresponding locus of a recipient strain using P1 bacteriophage transduction.
06:05
Nuclear Magnetic Resonance to Study Atomic Level Protein-Protein Interactions
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2025
This video describes the nuclear magnetic resonance spectroscopy technique to study protein-protein interactions between 15N-labeled wild-type and mutant envoplakin proteins and the unlabeled vimentin protein. The successful interaction between wild-type envoplakin and vimentin leads to extensive line broadening and peak disappearance in the NMR spectra, whereas the absence of an interaction between the mutated envoplakin and vimentin results in well-resolved peaks in the NMR spectra.
07:35
Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone
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Cited by 15 •
2017
This protocol describes the recovery of infectious Zika virus from a two-plasmid infectious cDNA clone.
03:04
An Intragastric Gavage Technique for Controlled Helicobacter Infection in Mice
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2025
This video demonstrates the establishment of Helicobacter pylori infection through the intragastric gavage technique in a mouse model. The bacterial suspension is administered via a catheter inserted through the mouth into the stomach. Inoculated bacteria colonize and establish an infection in the stomach, which can be used to study the host-pathogen interaction.
FAQs
Specificity arises from the interaction between P1 adhesin and sialylated molecules on host-cell membranes. These molecules provide the binding sites that connect Mycoplasma pneumoniae with ciliated epithelial cells in the respiratory tract. Because the protein is anchored at the organism’s specialized attachment organelle, this recognition is positioned where bacterial contact with the host surface can support colonization.
Accessory adhesins reinforce the attachment process rather than leaving surface binding dependent on P1 adhesin alone. Together, these proteins help Mycoplasma pneumoniae remain associated with ciliated respiratory cells despite mucociliary movement, which normally helps clear material from the airways. Their cooperation therefore contributes to persistence at the epithelial surface and supports the early stages of infection.
Attachment creates the physical association required for colonization of the respiratory epithelium, so changes in this interaction can influence how infection begins. The interaction is also relevant to tissue damage, making P1 adhesin useful for studying links between bacterial adhesion and disease development. This places the protein at the intersection of host-cell binding, persistence, and respiratory pathology.
P1 adhesin can serve as a target in diagnostic assays because it is a major surface protein associated with the organism’s attachment function. Assays directed toward this target can support investigations of Mycoplasma pneumoniae and its interaction with respiratory tissues. Its surface location and disease relevance make it a scientifically useful feature to examine when developing detection approaches.
Its exposed position on the bacterial surface and central role in host-cell attachment make P1 adhesin relevant to vaccine research. Studying the protein can help investigators examine whether immune-focused strategies might interfere with the interaction that supports colonization. This research connects a defined bacterial surface component with efforts to prevent the establishment or progression of respiratory infection.
Antimicrobial strategies that block P1 adhesin-mediated attachment can be used to investigate whether preventing bacterial binding reduces colonization-related effects. Such approaches focus on the host-pathogen interaction rather than only on the presence of the organism. In biology research, they help test the importance of adhesion for infection initiation and clarify how attachment relates to subsequent tissue damage.