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Neuroscience

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Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches
 

Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches

Article doi: 10.3791/60822
May 23rd, 2020

Summary May 23rd, 2020

In this article, we describe, in detail, a protocol for the generation of neurosphere cultures from postnatal mouse neural stem cells derived from the main mouse neurogenic niches. Neurospheres are used to identify neural stem cells from brain tissue allowing the estimation of precursor cell numbers. Moreover, these 3D structures can be plated in differentiative conditions, giving rise to neurons, oligodendrocytes and astrocytes, allowing the study of cell fate.

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