Siderophore Production
Siderophore Production - Related Videos
03:46
Isolation of Siderophore-Containing Extracellular Vesicles from Mycobacterium tuberculosis
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2025
Source: Gupta, S., Marcela Rodriguez, G. Isolation and Characterization of Extracellular Vesicles Produced by Iron-limited Mycobacteria. J. Vis. Exp. (2019)This video demonstrates the stepwise isolation of extracellular vesicles (EVs) from Mycobacterium tuberculosis cultured under iron-limiting conditions that mimic the host environment. It outlines the use of ultrafiltration, differential centrifugation, and density gradient separation to enrich and identify EV-containing fractions.
05:09
Qualitative and Quantitative Analysis of Siderophore Production from Pseudomonas aeruginosa
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Cited by 2 •
2024
This protocol provides both qualitative and quantitative analyses of total siderophores, pyoverdine, and pyochelin from Pseudomonas aeruginosa.
15:03
Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection
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2020
This work describes protocols for the preparation of magnetic nanoparticles, its coating with SiO2, followed by its amine functionalization with (3-aminopropyl)triethoxysilane (APTES) and its conjugation with deferoxamine using a succinyl moiety as a linker. A deep structural characterization description and a capture bacteria assay using Y. enterocolitica for all the intermediate nanoparticles and the final conjugate are also described in detail.
12:36
High-throughput Siderophore Screening from Environmental Samples: Plant Tissues, Bulk Soils, and Rhizosphere Soils
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Cited by 8 •
2019
We present a protocol for rapid screening of environmental samples for siderophore potential contributing to micronutrient bioavailability and turnover in terrestrial systems.
11:42
Lentivirus Production
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Cited by 43 •
2009
To make lentiviruses, DNA vectors are transfected into human 293 cells. After harvest and concentrating the supernatant, virus titer is determined by fluorescence expression with a flow cytometer.
FAQs
After binding ferric iron, a siderophore-iron complex must be recognized by a specific cellular receptor before transport across the cell envelope can occur. This recognition step links environmental iron capture to selective uptake, helping determine whether a microorganism can convert extracellular iron into a usable resource for growth.
In iron-limited surroundings, strong ferric-iron binding can give a microorganism access to iron that would otherwise be difficult to obtain. The advantage is biological rather than merely chemical: successful capture and receptor-mediated uptake can support growth, while differences in access may influence which microbes persist when they share the same environment.
Changes in available iron can reshape a microbial community because organisms differ in their ability to acquire and use this resource. Siderophore production therefore has consequences beyond an individual cell: it can affect competitive relationships and help explain why particular microorganisms become more or less successful under iron-limited conditions.
Studying siderophore production can connect iron acquisition with broader biological patterns. In microbial ecology, it can help researchers examine nutrient cycling and community composition. In host-associated systems, the same information can contribute to understanding how iron availability relates to interactions between microorganisms and hosts, including variation in infection outcomes.
Siderophore production matters in host-microbe interactions because iron availability can shape the outcome of those relationships. A microorganism able to capture iron through the siderophore-receptor-transport pathway may gain an ecological advantage in a host-associated environment. For research, this makes iron uptake relevant to examining infection and considering ways to control pathogens.
In beneficial plant-microbe associations, studying siderophore production can clarify how iron acquisition contributes to the relationship. This knowledge supports research aimed at improving such associations, while also informing strategies that target microbial iron uptake to control pathogens. The same process therefore connects agricultural interests with broader questions about nutrient availability and microbial behavior.