Ultraviolet Light Degradation
Ultraviolet Light Degradation - Related Videos
03:07
Targeted Bacterial Colony Isolation Using Light-Patterned Hydrogel Degradation
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2025
Source: Fattahi, N., et al. Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation. J. Vis. Exp. (2021).This video demonstrates the extraction of bacterial colonies from a photodegradable hydrogel using UV light. A specific colony is selected, and a patterned light exposure is designed to initiate localized hydrogel degradation. After adjusting light intensity and duration, the UV light is applied, releasing the colony. The colony is then aspirated through tubing...
09:21
Ultraviolet-Visible (UV-Vis) Spectroscopy
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2023
Source: Laboratory of Dr. B. Jill Venton - University of Virginia Ultraviolet-visible (UV-Vis) spectroscopy is one of the most popular analytical techniques because it is very versatile and able to detect nearly every molecule. With UV-Vis spectroscopy, the UV-Vis light is passed through a sample and the transmittance of light by a sample is measured. From the transmittance (T), the absorbance can be calculated as A=-log (T). An absorbance spectrum is obtained that shows the absorbance of a...
02:58
Regulated Protein Degradation
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2020
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells. Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
02:11
Proteins: From Genes to Degradation
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2020
Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick. Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation. Transcription is the synthesis of RNA molecules by RNA...
07:43
Assaying Proteasomal Degradation in a Cell-free System in Plants
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Cited by 26 •
2014
Targeted protein degradation represents a major regulatory mechanism for cell function. It occurs via a conserved ubiquitin-proteasome pathway, which attaches polyubiquitin chains to the target protein that then serve as molecular “tags” for the 26S proteasome. Here, we describe a simple and reliable cell-free assay for proteasomal degradation of proteins.
FAQs
Cyclobutane pyrimidine dimers matter because UV photons can alter nucleic acids after DNA absorbs the radiation. These lesions provide a direct molecular link between UV exposure and damaged genetic material. Their formation is especially important in studies of mutation and carcinogenesis, where researchers examine how radiation-associated DNA changes contribute to broader biological consequences.
Reactive oxygen species extend UV damage beyond nucleic acids. Indirect photochemical reactions can generate these chemically reactive molecules, which oxidize proteins, lipids, and other cellular components. This creates a broader pattern of cellular stress than direct DNA absorption alone and helps explain why UV exposure can affect multiple types of biological molecules simultaneously.
Protection operates at several levels. Protective pigments can limit the effects of incoming radiation, while antioxidant defenses help counter oxidative damage caused by reactive oxygen species. DNA-repair pathways, including nucleotide excision repair, address certain radiation-induced lesions in genetic material. Studying these defenses reveals how organisms reduce molecular injury rather than relying on one protective mechanism.
Researchers can compare direct DNA lesions with oxidative changes in proteins, lipids, and other cellular components to characterize different consequences of exposure. They can also examine how protective defenses and nucleotide excision repair respond. Together, these observations connect molecular damage with research questions involving mutation, carcinogenesis, aging, and cellular responses to environmental stress.
UV-related molecular damage has practical relevance in microbial control because radiation can affect essential biological components in microorganisms. The same subject also supports photosensitivity research, which examines biological responses associated with heightened sensitivity to light. These applications extend the topic beyond DNA damage alone and show how UV effects can be studied across different biological systems.
Solar radiation exposes living systems to an environmental source of UV stress, making its biological effects relevant outside controlled laboratory studies. Research can connect this exposure with molecular damage, aging, and the capacity of organisms to protect or repair affected components. This perspective helps place UV-induced changes within broader studies of environmental stress and organismal biology.