Macs Express Separator
Macs Express Separator - Related Videos
11:35
Magnetic Activated Cell Sorting (MACS): Isolation of Thymic T Lymphocytes
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2023
Source: Meunier Sylvain1,2,3, Perchet Thibaut1,2,3, Sophie Novault4, Rachel Golub1,2,3 1 Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, Paris, France 2 INSERM U1223, Paris, France 3 Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France 4 Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute, Paris, France Defence against pathogens depends on surveillance by the immune system. This system is...
03:57
T-Cell Enrichment: A Technique to Isolate T-Cells from Mixed Cell Population by Magnetic Separation
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2023
This video describes T-cells enrichment from the spleen of a mouse by negative selection using a magnetic separator. The technique involves targeting the other cells in the sample for depletion using antibodies or ligands directed against specific cell surface antigens.
09:47
Isolation of CD4+ T cells from Mouse Lymph Nodes Using Miltenyi MACS Purification
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Cited by 19 •
2007
Isolation of lymphocytes using the Miltenyi MACs kit is a reliable way to purify cells from whole lymphoid tissue homogenates. Cells purified using the Miltenyi system are typically ≥ 96% pure. Here, we demonstrate the steps taken to isolate CD4+ T cells, one of the many kits offered by Miltenyi.
08:28
Subretinal Transplantation of MACS Purified Photoreceptor Precursor Cells into the Adult Mouse Retina
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Cited by 43 •
2014
Cell transplantation represents a strategy for the treatment of retinal degeneration characterized by photoreceptor loss. Here we describe a method for enrichment of transplantable photoreceptors and their subretinal grafting into adult mice.
08:02
Separation of Mixtures via Precipitation
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2023
Source: Laboratory of Dr. Ana J. García-Sáez — University of Tübingen Most samples of interest are mixtures of many different components. Sample preparation, a key step in the analytical process, removes interferences that may affect the analysis. As such, developing separation techniques is an important endeavor not just in academia, but also in industry. One way to separate mixtures is to use their solubility properties. In this short paper, we will deal with aqueous solutions. The...
FAQs
Antibody-conjugated magnetic microbeads create a selective link between labeled cells and the separation column. During loading, the magnetic field retains these cells, whereas unlabeled cells pass through. When the column is removed from the field, the retained population can be released as an enriched fraction, allowing researchers to distinguish target cells from surrounding material.
Microbead labeling identifies the population that the magnetic field will retain. Because the beads are attached through antibodies, the selected fraction depends on which cellular population is targeted during sample preparation. This enables enrichment of tumor cells, immune-cell subsets, or other rare populations from complex cancer research specimens rather than processing the entire mixture uniformly.
The benchtop instrument streamlines magnetic separation so samples can be handled with a consistent workflow. Its rapid and gentle operation supports reproducible preparation while reducing variation between specimens. This consistency is particularly relevant when researchers compare enriched tumor or immune-cell populations across multiple samples for downstream analyses or functional experiments.
The workflow begins by labeling the chosen cells with antibody-conjugated magnetic microbeads. The prepared sample then passes through a separation column in the instrument, where the magnetic field retains labeled cells and unlabeled cells flow through. Removing the column from the field releases the retained fraction for collection and subsequent study.
Cancer researchers can apply the approach to tumor cells, immune-cell subsets, and other rare populations present in complex specimens. Enrichment concentrates the selected cells before analysis, which can help investigators work with a defined population rather than a mixed starting sample. The relevant target depends on the antibody used for microbead labeling.
The recovered population can support flow cytometry, molecular profiling, functional assays, and downstream culture. Separating the target cells before these studies helps align the experimental material with the research question, whether investigators are characterizing tumor or immune populations, examining molecular features, assessing function, or maintaining cells for further work.