Density Method
Density Method - Related Videos
08:14
Rapid Magnetic-microbead Method for Efficient Purification of Low-density Neutrophils
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Cited by 1 •
2025
Low-density neutrophils (LDN) increase significantly in several diseases. LDN are usually isolated through cell sorting. We present a practical method to obtain pure and viable LDN. After density-gradient centrifugation of peripheral blood, cells are incubated with magnetic microbeads, and then LDN are separated through magnetic columns.
09:48
Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein
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Cited by 2 •
2016
MicroRNAs play an important regulatory role and are emerging as novel therapeutic targets for various human diseases. It has been shown that miRNAs are carried in high density lipoproteins. We have developed a simplified method to rapidly isolate purified HDL suitable for miRNA analysis from human plasma.
11:19
A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture
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Cited by 45 •
2016
Low density cultures of primary hippocampal neurons usually require glia feeder layer to supply neurotrophic factors and sustain longevity. We describe here a simplified method to culture ultra-low density neurons on glass coverslips in the presence of a high density neuronal feeder layer, which facilitates investigation of specific neuronal-autonomous mechanisms.
03:40
Density Gradient Centrifugation: A Method to Isolate CLL Cells from Peripheral Blood
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2023
This video describes the technique of isolating chronic lymphocytic leukemia (CLL) cells from peripheral blood. With this protocol, we will isolate CLL cells from human patient blood sample for subsequent efficient generation of nuclear and cytoplasmic fractions.
11:02
Quantifying Branching Density in Rat Mammary Gland Whole-mounts Using the Sholl Analysis Method
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Cited by 29 •
2017
Mammary gland development in the rodent has typically been evaluated using descriptive assessments or by measuring basic physical attributes. Branching density is an indicator of mammary development that is difficult to quantify objectively. This protocol describes a reliable method for the quantitative assessment of mammary gland branching characteristics.
FAQs
Temperature control helps ensure that changes in measured density reflect the sample rather than uncontrolled thermal effects. Because volume can change as temperature changes, a value obtained under one condition may not be directly comparable with a value obtained under another. In chemistry, consistent temperature conditions strengthen comparisons and improve assessment of composition, purity, or process behavior.
A hydrometer uses buoyancy to relate how deeply it floats to a liquid’s density. A denser liquid provides greater buoyant support, so the instrument’s position changes accordingly. This makes the device useful for examining liquids and solutions, especially when repeated readings are needed under controlled temperature conditions.
For a solid whose volume is not readily established from its dimensions, measuring the liquid volume displaced by the sample offers an alternative. The method compares the liquid level before and after immersion, while mass is measured separately. This approach supports density determination for solids and can aid material identification or phase characterization.
Measure the mass and volume of the same sample, keeping the measurement conditions consistent, and use those values together in the density calculation. For liquid work, a hydrometer may provide the density-related reading directly through buoyancy. Careful matching of sample, volume, and temperature helps prevent misleading comparisons.
By comparing measured density values across samples or against expected values, analysts can assess composition and purity and identify concentration-related changes in solutions. The result provides a measurable property for quality control, allowing laboratories to compare materials and evaluate whether sample composition remains consistent during testing.
Tracking density over time or across samples can reveal changes associated with chemical processing, composition, or phase characterization. Researchers can use these measurements to compare materials and evaluate whether a process remains consistent. This makes density data useful in chemistry research and quality control, not only in one-time material identification.