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JoVE Journal
Neuroscience

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A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture
 

A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture

Article DOI: 10.3791/53797
March 5th, 2016

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Summary March 5th, 2016

Low density cultures of primary hippocampal neurons usually require glia feeder layer to supply neurotrophic factors and sustain longevity. We describe here a simplified method to culture ultra-low density neurons on glass coverslips in the presence of a high density neuronal feeder layer, which facilitates investigation of specific neuronal-autonomous mechanisms.

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